20 alpha-hydroxysteroid dehydrogenase expression in hemopoietic cell cultures and its relationship to interleukin 3.
| dc.contributor.author | Garland, John M | |
| dc.contributor.author | Dexter, T Michael | |
| dc.date.accessioned | 2011-04-17T08:49:17Z | |
| dc.date.available | 2011-04-17T08:49:17Z | |
| dc.date.issued | 1982-12 | |
| dc.identifier.citation | 20 alpha-hydroxysteroid dehydrogenase expression in hemopoietic cell cultures and its relationship to interleukin 3. 1982, 12 (12):998-1001 Eur J Immunol | en |
| dc.identifier.issn | 0014-2980 | |
| dc.identifier.pmid | 6819151 | |
| dc.identifier.doi | 10.1002/eji.1830121203 | |
| dc.identifier.uri | http://hdl.handle.net/10541/128210 | |
| dc.description.abstract | Expression of 20 alpha-hydroxysteroid dehydrogenase (20 alpha-SDH) has been investigated in long-term bone marrow cultures derived from normal and nude mice in the presence of horse serum and hydrocortisone. 20 alpha-SDH expression rises markedly in the adherent cells derived from normal bone marrow. Early (1-5 days) lipid profiles are similar to those seen in T cells, but at later times (6-14 days) stromal-type lipid patterns dominate. Similar culture of fetal liver and nu/nu bone marrow cells shows rises in 20 alpha-SDH expression, in situations where T cells are notably absent. The expression of 20 alpha-SDH is not associated with detectable endogenous production of "interleukin 3" activity, and although addition of "IL 3" to bone marrow cultures increases the levels of 20 alpha-SDH, this is associated with granulopoiesis rather than lymphoid development. | |
| dc.language.iso | en | en |
| dc.subject | Foestus | en |
| dc.subject | Haematopoietic Stem Cells | en |
| dc.subject.mesh | 20-Hydroxysteroid Dehydrogenases | |
| dc.subject.mesh | Animals | |
| dc.subject.mesh | Bone Marrow | |
| dc.subject.mesh | Cells, Cultured | |
| dc.subject.mesh | Fetus | |
| dc.subject.mesh | Granulocytes | |
| dc.subject.mesh | Hematopoietic Stem Cells | |
| dc.subject.mesh | Interleukin-3 | |
| dc.subject.mesh | Liver | |
| dc.subject.mesh | Lymphokines | |
| dc.subject.mesh | Mice | |
| dc.subject.mesh | Mice, Nude | |
| dc.subject.mesh | Spleen | |
| dc.subject.mesh | T-Lymphocytes | |
| dc.title | 20 alpha-hydroxysteroid dehydrogenase expression in hemopoietic cell cultures and its relationship to interleukin 3. | en |
| dc.type | Article | en |
| dc.contributor.department | Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester, M20 9BX, UK | en |
| dc.identifier.journal | European journal of immunology | en |
| html.description.abstract | Expression of 20 alpha-hydroxysteroid dehydrogenase (20 alpha-SDH) has been investigated in long-term bone marrow cultures derived from normal and nude mice in the presence of horse serum and hydrocortisone. 20 alpha-SDH expression rises markedly in the adherent cells derived from normal bone marrow. Early (1-5 days) lipid profiles are similar to those seen in T cells, but at later times (6-14 days) stromal-type lipid patterns dominate. Similar culture of fetal liver and nu/nu bone marrow cells shows rises in 20 alpha-SDH expression, in situations where T cells are notably absent. The expression of 20 alpha-SDH is not associated with detectable endogenous production of "interleukin 3" activity, and although addition of "IL 3" to bone marrow cultures increases the levels of 20 alpha-SDH, this is associated with granulopoiesis rather than lymphoid development. |