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dc.contributor.authorSaffhill, Royen
dc.contributor.authorStrickland, Pen
dc.contributor.authorBoyle, John Men
dc.date.accessioned2011-03-22T17:17:41Z
dc.date.available2011-03-22T17:17:41Z
dc.date.issued1982
dc.identifier.citationSensitive radioimmunoassays for O6-n-butyldeoxyguanosine, O2-n-butylthymidine and O4-n-butylthymidine. 1982, 3 (5):547-52 Carcinogenesisen
dc.identifier.issn0143-3334
dc.identifier.pmid7094213
dc.identifier.doi10.1093/carcin/3.5.547
dc.identifier.urihttp://hdl.handle.net/10541/125376
dc.description.abstractRadioimmunoassays have been developed using monoclonal antibodies from hybridomas raised against bovine serum albumin conjugates of O6-n-butylguanosine, O2-n-butylthymidine riboside and O4-n-butylthymidine riboside. The assays showed 50% inhibition of binding of specific (( 3H]butyl-deoxynucleosides by 0.044, 0.069 and 0.45 pmole of cold O6-n-butyldeoxyguanosine, O2-n-butylthymidine and O4-n-butylthymidine respectively, corresponding to affinity constants of 2.7 x 10(10), 1.1 x 10(10) and 8.8 x 10(8) respectively. In competitive radioimmunoassays a similar degree of inhibition required approximately 10(7)-fold higher concentrations of normal deoxynucleotides. From the relative inhibitions produced by a wide range of alkylated and normal nucleosides and bases we conclude that each antibody primarily recognises butylation, as compared to other alkylations, at the sites specified by the immunogen. The radioimmunoassays should be suitable for the detection of these potentially promutagenic lesions in digests of DNA exposed to low (biological) levels of butylation.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAntibodies, Monoclonal
dc.subject.meshAntibody Affinity
dc.subject.meshAntibody Specificity
dc.subject.meshDeoxyguanosine
dc.subject.meshGuanosine
dc.subject.meshMice
dc.subject.meshMice, Inbred BALB C
dc.subject.meshRadioimmunoassay
dc.subject.meshThymidine
dc.titleSensitive radioimmunoassays for O6-n-butyldeoxyguanosine, O2-n-butylthymidine and O4-n-butylthymidine.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, M20 9BX, United Kingdomen
dc.identifier.journalCarcinogenesisen
html.description.abstractRadioimmunoassays have been developed using monoclonal antibodies from hybridomas raised against bovine serum albumin conjugates of O6-n-butylguanosine, O2-n-butylthymidine riboside and O4-n-butylthymidine riboside. The assays showed 50% inhibition of binding of specific (( 3H]butyl-deoxynucleosides by 0.044, 0.069 and 0.45 pmole of cold O6-n-butyldeoxyguanosine, O2-n-butylthymidine and O4-n-butylthymidine respectively, corresponding to affinity constants of 2.7 x 10(10), 1.1 x 10(10) and 8.8 x 10(8) respectively. In competitive radioimmunoassays a similar degree of inhibition required approximately 10(7)-fold higher concentrations of normal deoxynucleotides. From the relative inhibitions produced by a wide range of alkylated and normal nucleosides and bases we conclude that each antibody primarily recognises butylation, as compared to other alkylations, at the sites specified by the immunogen. The radioimmunoassays should be suitable for the detection of these potentially promutagenic lesions in digests of DNA exposed to low (biological) levels of butylation.


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