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    Sensitive radioimmunoassays for O6-n-butyldeoxyguanosine, O2-n-butylthymidine and O4-n-butylthymidine.

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    Authors
    Saffhill, Roy
    Strickland, P
    Boyle, John M
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, M20 9BX, United Kingdom
    Issue Date
    1982
    
    Metadata
    Show full item record
    Abstract
    Radioimmunoassays have been developed using monoclonal antibodies from hybridomas raised against bovine serum albumin conjugates of O6-n-butylguanosine, O2-n-butylthymidine riboside and O4-n-butylthymidine riboside. The assays showed 50% inhibition of binding of specific (( 3H]butyl-deoxynucleosides by 0.044, 0.069 and 0.45 pmole of cold O6-n-butyldeoxyguanosine, O2-n-butylthymidine and O4-n-butylthymidine respectively, corresponding to affinity constants of 2.7 x 10(10), 1.1 x 10(10) and 8.8 x 10(8) respectively. In competitive radioimmunoassays a similar degree of inhibition required approximately 10(7)-fold higher concentrations of normal deoxynucleotides. From the relative inhibitions produced by a wide range of alkylated and normal nucleosides and bases we conclude that each antibody primarily recognises butylation, as compared to other alkylations, at the sites specified by the immunogen. The radioimmunoassays should be suitable for the detection of these potentially promutagenic lesions in digests of DNA exposed to low (biological) levels of butylation.
    Citation
    Sensitive radioimmunoassays for O6-n-butyldeoxyguanosine, O2-n-butylthymidine and O4-n-butylthymidine. 1982, 3 (5):547-52 Carcinogenesis
    Journal
    Carcinogenesis
    URI
    http://hdl.handle.net/10541/125376
    DOI
    10.1093/carcin/3.5.547
    PubMed ID
    7094213
    Type
    Article
    Language
    en
    ISSN
    0143-3334
    ae974a485f413a2113503eed53cd6c53
    10.1093/carcin/3.5.547
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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