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    Mitogen responsiveness and inhibitory activity of mesenteric lymph node cells. Conditioned medium containing T cell growth factor reverses suppressor function.

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    Authors
    Vose, Brent M
    Ferguson, R
    Moore, Michael
    Affiliation
    Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital & Holt Radium Institute, Manchester, United Kingdom.
    Issue Date
    1982
    
    Metadata
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    Abstract
    Blood-, lymph node-, and tumour-infiltrating lymphocytes (PBL, LNC, and TIL, respectively) from patients with colonic neoplasms were tested for responsiveness to phytohaemagglutinin (PHA). All populations responded, with LNC and PBL showing comparable reactivities while TIL were less reactive as assessed by incorporation of 3H-thymidine. Increased mitogen responsiveness was observed for T cells enriched by SRBC rosette formation or passage through nylon columns. Mitomycin C-treated LNC and TIL inhibited PHA induced 3H-thymidine incorporation of admixed autologous PBL, suggesting the presence of suppressor cells. Suppressor activity resided primarily in the SRBC rosetting population and was dose-dependent, with increasing numbers of LNC giving greater diminution of PHA response. Suppression by LNC was apparent only when they were added to PBL responders within 6 h of the initiation of stimulation assays, in common with the effects of Concanavalin A (Con A)-induced suppressors on PBL phytomitogen responsiveness. Con A-induced and LNC-suppressor activity could be reversed by addition of lymphocyte-conditioned medium (CM) containing T cell growth factor (TCGF; interleukin IL-2). These data provide further evidence that the suppressor phenomena observed in this system are a function of activated T cells present both in drainage lymph nodes and at the tumour site.
    Citation
    Mitogen responsiveness and inhibitory activity of mesenteric lymph node cells. Conditioned medium containing T cell growth factor reverses suppressor function. 1982, 13 (2):105-11 Cancer Immunol Immunother
    Journal
    Cancer Immunology, Immunotherapy
    URI
    http://hdl.handle.net/10541/125373
    DOI
    10.1007/BF00205309
    PubMed ID
    6218870
    Type
    Article
    Language
    en
    ISSN
    0340-7004
    ae974a485f413a2113503eed53cd6c53
    10.1007/BF00205309
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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