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dc.contributor.authorBedford, P
dc.contributor.authorFox, Brian W
dc.date.accessioned2011-03-22T17:09:04Z
dc.date.available2011-03-22T17:09:04Z
dc.date.issued1982
dc.identifier.citationRepair of DNA interstrand crosslinks after busulphan. A possible mode of resistance. 1982, 8 (1):3-7 Cancer Chemother Pharmacolen
dc.identifier.issn0344-5704
dc.identifier.pmid7094199
dc.identifier.doi10.1007/BF00292863
dc.identifier.urihttp://hdl.handle.net/10541/125372
dc.description.abstractThe technique of alkaline elution has been used to study the interaction between the antineoplastic drug busulphan and the DNA of cells derived from the transplantable rodent Yoshida sarcoma. A dose-dependent proteinase-resistant filter retention was observed after drug treatment, which indicated the presence of DNA interstrand cross-links. Such cross-links were removed after 6 h in cells resistant to busulphan but not in the busulphan-sensitive parent cells, even after 24 h. Such temporal differences in DNA cross-linking could be correlated with cell survival and also with the level of anaphase chromosome aberrations, which was found to be four-fold higher in the sensitive line than in the resistant line.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBusulfan
dc.subject.meshChromosome Aberrations
dc.subject.meshDNA Repair
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshDrug Resistance
dc.subject.meshFemale
dc.subject.meshRats
dc.subject.meshRats, Inbred Strains
dc.subject.meshSarcoma, Yoshida
dc.titleRepair of DNA interstrand crosslinks after busulphan. A possible mode of resistance.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, United Kingdomen
dc.identifier.journalCancer Chemotherapy and Pharmacologyen
html.description.abstractThe technique of alkaline elution has been used to study the interaction between the antineoplastic drug busulphan and the DNA of cells derived from the transplantable rodent Yoshida sarcoma. A dose-dependent proteinase-resistant filter retention was observed after drug treatment, which indicated the presence of DNA interstrand cross-links. Such cross-links were removed after 6 h in cells resistant to busulphan but not in the busulphan-sensitive parent cells, even after 24 h. Such temporal differences in DNA cross-linking could be correlated with cell survival and also with the level of anaphase chromosome aberrations, which was found to be four-fold higher in the sensitive line than in the resistant line.


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