Repair of DNA interstrand crosslinks after busulphan. A possible mode of resistance.
dc.contributor.author | Bedford, P | |
dc.contributor.author | Fox, Brian W | |
dc.date.accessioned | 2011-03-22T17:09:04Z | |
dc.date.available | 2011-03-22T17:09:04Z | |
dc.date.issued | 1982 | |
dc.identifier.citation | Repair of DNA interstrand crosslinks after busulphan. A possible mode of resistance. 1982, 8 (1):3-7 Cancer Chemother Pharmacol | en |
dc.identifier.issn | 0344-5704 | |
dc.identifier.pmid | 7094199 | |
dc.identifier.doi | 10.1007/BF00292863 | |
dc.identifier.uri | http://hdl.handle.net/10541/125372 | |
dc.description.abstract | The technique of alkaline elution has been used to study the interaction between the antineoplastic drug busulphan and the DNA of cells derived from the transplantable rodent Yoshida sarcoma. A dose-dependent proteinase-resistant filter retention was observed after drug treatment, which indicated the presence of DNA interstrand cross-links. Such cross-links were removed after 6 h in cells resistant to busulphan but not in the busulphan-sensitive parent cells, even after 24 h. Such temporal differences in DNA cross-linking could be correlated with cell survival and also with the level of anaphase chromosome aberrations, which was found to be four-fold higher in the sensitive line than in the resistant line. | |
dc.language.iso | en | en |
dc.subject.mesh | Animals | |
dc.subject.mesh | Busulfan | |
dc.subject.mesh | Chromosome Aberrations | |
dc.subject.mesh | DNA Repair | |
dc.subject.mesh | Dose-Response Relationship, Drug | |
dc.subject.mesh | Drug Resistance | |
dc.subject.mesh | Female | |
dc.subject.mesh | Rats | |
dc.subject.mesh | Rats, Inbred Strains | |
dc.subject.mesh | Sarcoma, Yoshida | |
dc.title | Repair of DNA interstrand crosslinks after busulphan. A possible mode of resistance. | en |
dc.type | Article | en |
dc.contributor.department | Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, United Kingdom | en |
dc.identifier.journal | Cancer Chemotherapy and Pharmacology | en |
html.description.abstract | The technique of alkaline elution has been used to study the interaction between the antineoplastic drug busulphan and the DNA of cells derived from the transplantable rodent Yoshida sarcoma. A dose-dependent proteinase-resistant filter retention was observed after drug treatment, which indicated the presence of DNA interstrand cross-links. Such cross-links were removed after 6 h in cells resistant to busulphan but not in the busulphan-sensitive parent cells, even after 24 h. Such temporal differences in DNA cross-linking could be correlated with cell survival and also with the level of anaphase chromosome aberrations, which was found to be four-fold higher in the sensitive line than in the resistant line. |