Repair of DNA interstrand crosslinks after busulphan. A possible mode of resistance.
AffiliationPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, United Kingdom
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AbstractThe technique of alkaline elution has been used to study the interaction between the antineoplastic drug busulphan and the DNA of cells derived from the transplantable rodent Yoshida sarcoma. A dose-dependent proteinase-resistant filter retention was observed after drug treatment, which indicated the presence of DNA interstrand cross-links. Such cross-links were removed after 6 h in cells resistant to busulphan but not in the busulphan-sensitive parent cells, even after 24 h. Such temporal differences in DNA cross-linking could be correlated with cell survival and also with the level of anaphase chromosome aberrations, which was found to be four-fold higher in the sensitive line than in the resistant line.
CitationRepair of DNA interstrand crosslinks after busulphan. A possible mode of resistance. 1982, 8 (1):3-7 Cancer Chemother Pharmacol
JournalCancer Chemotherapy and Pharmacology