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dc.contributor.authorSwinburne, S
dc.contributor.authorMoore, Michael
dc.contributor.authorCole, P
dc.date.accessioned2011-03-22T17:08:32Z
dc.date.available2011-03-22T17:08:32Z
dc.date.issued1982-10
dc.identifier.citationHuman bronchoalveolar macrophage cytotoxicity for cultured human lung-tumour cells. 1982, 46 (4):625-34 Br J Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid7138768
dc.identifier.urihttp://hdl.handle.net/10541/125371
dc.description.abstractHuman bronchoalveolar macrophages were separated from other free lung cells by density sedimentation on Percoll gradients. They were then tested for cytotoxicity against the human lung adenocarcinoma cell line A549, using a Selenomethionine-75 post-labelling assay. The cytotoxicity of the macrophages increased as the effector:target cell ratio was increased, approaching 100% at 20:1. There was no significant difference in the cytotoxicity of macrophages isolated from the lungs of bronchial-carcinoma or non-carcinoma patients. The highly cytotoxic nature of the macrophages was not due to selection of a more potent cytotoxic subpopulation of macrophages on the Percoll gradient, nor to a generally elevated activation of the macrophages due to the pathological conditions in the patients' lungs. An attempt to determine whether low concentrations of macrophages could potentiate target-cell growth proved negative. Cytotoxicity of macrophages for cultured lung target cells was not restricted to A549 cells and is not in accordance with the view that defective bronchoalveolar macrophage cytotoxicity contributes to the emergence of bronchial neoplasia.
dc.language.isoenen
dc.subjectLung Canceren
dc.subject.meshAdenocarcinoma
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshBronchi
dc.subject.meshCarcinoma, Bronchogenic
dc.subject.meshCell Line
dc.subject.meshCell Separation
dc.subject.meshCytotoxicity, Immunologic
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshLung
dc.subject.meshLung Diseases
dc.subject.meshLung Neoplasms
dc.subject.meshLymphocytes
dc.subject.meshMacrophages
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshNeutrophils
dc.subject.meshPulmonary Alveoli
dc.titleHuman bronchoalveolar macrophage cytotoxicity for cultured human lung-tumour cells.en
dc.typeArticleen
dc.contributor.departmentHost Defence Unit, Deaprtment of Medicine, Cardiothoraci Institute, Brompton Hospital, Fulham Road, London SW3 6HP.en
dc.identifier.journalBritish Journal of Canceren
html.description.abstractHuman bronchoalveolar macrophages were separated from other free lung cells by density sedimentation on Percoll gradients. They were then tested for cytotoxicity against the human lung adenocarcinoma cell line A549, using a Selenomethionine-75 post-labelling assay. The cytotoxicity of the macrophages increased as the effector:target cell ratio was increased, approaching 100% at 20:1. There was no significant difference in the cytotoxicity of macrophages isolated from the lungs of bronchial-carcinoma or non-carcinoma patients. The highly cytotoxic nature of the macrophages was not due to selection of a more potent cytotoxic subpopulation of macrophages on the Percoll gradient, nor to a generally elevated activation of the macrophages due to the pathological conditions in the patients' lungs. An attempt to determine whether low concentrations of macrophages could potentiate target-cell growth proved negative. Cytotoxicity of macrophages for cultured lung target cells was not restricted to A549 cells and is not in accordance with the view that defective bronchoalveolar macrophage cytotoxicity contributes to the emergence of bronchial neoplasia.


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