Human bronchoalveolar macrophage cytotoxicity for cultured human lung-tumour cells.
AffiliationHost Defence Unit, Deaprtment of Medicine, Cardiothoraci Institute, Brompton Hospital, Fulham Road, London SW3 6HP.
MetadataShow full item record
AbstractHuman bronchoalveolar macrophages were separated from other free lung cells by density sedimentation on Percoll gradients. They were then tested for cytotoxicity against the human lung adenocarcinoma cell line A549, using a Selenomethionine-75 post-labelling assay. The cytotoxicity of the macrophages increased as the effector:target cell ratio was increased, approaching 100% at 20:1. There was no significant difference in the cytotoxicity of macrophages isolated from the lungs of bronchial-carcinoma or non-carcinoma patients. The highly cytotoxic nature of the macrophages was not due to selection of a more potent cytotoxic subpopulation of macrophages on the Percoll gradient, nor to a generally elevated activation of the macrophages due to the pathological conditions in the patients' lungs. An attempt to determine whether low concentrations of macrophages could potentiate target-cell growth proved negative. Cytotoxicity of macrophages for cultured lung target cells was not restricted to A549 cells and is not in accordance with the view that defective bronchoalveolar macrophage cytotoxicity contributes to the emergence of bronchial neoplasia.
CitationHuman bronchoalveolar macrophage cytotoxicity for cultured human lung-tumour cells. 1982, 46 (4):625-34 Br J Cancer
JournalBritish Journal of Cancer
- Human peripheral blood monocyte and bronchoalveolar macrophage cytotoxicity for cultured human lung tumor cells.
- Authors: Swinburne S, Cole P
- Issue date: 1982 Nov
- Bronchoalveolar lavage and the immunology of primary lung cancer.
- Authors: Olsen GN, Gangemi JD
- Issue date: 1985 May
- [Determination of macrophage activity in bronchoalveolar lavage fluid in patients with lung diseases].
- Authors: Ochendalski M, Müller S, Unger U, Grollmuss H, Schilling W
- Issue date: 1987
- Phagocytic activity of alveolar macrophages in patients with bronchogenic carcinoma.
- Authors: Gangemi JD, Olsen GN, Fechter C, Hightower JA, Bauguess CT, Krech L
- Issue date: 1985
- Lymphokine-activated killer cell activity in lung cancer.
- Authors: LeFever A, Funahashi A
- Issue date: 1991 Feb