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    Flow cytometric analysis of lectin binding to human peripheral blood lymphocytes.

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    Authors
    Blackledge, George
    Vose, Brent M
    Morris, Andrew J
    Crowther, Derek
    Gallagher, John T
    Affiliation
    Cancer Research Campaign, Department of Medical Oncology, Christie Hospital and Holt Radium Institute, UK.
    Issue Date
    1982-10-15
    
    Metadata
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    Abstract
    The binding of fluorescein-conjugated lentil lectin and concanavalin A to the surface membrane of human peripheral blood lymphocytes was studied by flow cytometry. The lymphocytes bound 3-fold more lentil lectin molecules compared with concanavalin A molecules and lentil lectin binding approached saturation at a much lower concentration than did that of concanavalin A. Lentil lectin identified two groups of lymphocytes: a low-binding T-cell fraction and a high-binding B-cell-enriched fraction. Concanavalin A did not discriminate between these populations in unseparated lymphocytes. Competition studies indicated that lentil lectin and concanavalin A were bound to different sites on the lymphocyte surface, although about 50% of lentil lectin sites were in close proximity to concanavalin A sites.
    Citation
    Flow cytometric analysis of lectin binding to human peripheral blood lymphocytes. 1982, 208 (1):69-75 Biochem J
    Journal
    The Biochemical Journal
    URI
    http://hdl.handle.net/10541/125354
    PubMed ID
    6818949
    Type
    Article
    Language
    en
    ISSN
    0264-6021
    Collections
    All Paterson Institute for Cancer Research

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