Continuous in vitro generation of multipotential stem cell clones from src-infected cultures.
Affiliation
Department of Experimental Haematology, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BX, UK.Issue Date
2011-03-13T00:13:42Z
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A molecular recombinant of Rous sarcoma virus and murine amphotropic leukaemia virus, src(MoMuLV), where the avian src oncogene has been placed under the influence of a murine virus promoter sequence, has been reported. Infection of long-term marrow cultures with this virus led to a dramatic change in the relative numbers of stem cells, granulocyte-macrophage progenitor cells and mature cells found in normal haematopoietic cell development. However, although the balance between self-renewal, differentiation and development was disturbed, injection of the cultured cells into irradiated syngeneic recipients did not lead to the development of leukaemia. Thus, although the control had been 'loosened', the host regulatory mechanisms were sufficient to impose a restraint on unlimited growth of the cells. We now show that the stem cells from the src-infected cultures show a remarkably increased capacity for self-renewal in vitro in situations which are inimical to the maintenance of self-renewal in normal uninfected stem cells and that self-renewal/differentiation can be modified by the culture conditions.Citation
Continuous in vitro generation of multipotential stem cell clones from src-infected cultures., 310 (5974):228-30 NatureJournal
NatureDOI
10.1038/310228a0PubMed ID
6146932Type
ArticleLanguage
enISSN
0028-0836ae974a485f413a2113503eed53cd6c53
10.1038/310228a0
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