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dc.contributor.authorGainer, H Sen
dc.contributor.authorSchor, Seth Len
dc.contributor.authorKinsella, Anne Ren
dc.date.accessioned2011-03-13T00:07:27Z
dc.date.available2011-03-13T00:07:27Z
dc.date.issued1984-09-15
dc.identifier.citationSusceptibility of skin fibroblasts from individuals genetically predisposed to cancer, to transformation by the tumour promoter 12-O-tetradecanoylphorbol-13-acetate. 1984, 34 (3):349-57 Int J Canceren
dc.identifier.issn0020-7136
dc.identifier.pmid6480155
dc.identifier.doi10.1002/ijc.2910340311
dc.identifier.urihttp://hdl.handle.net/10541/124429
dc.description.abstractSkin fibroblasts from patients with hereditary retinoblastoma (RB) and familial polyposis coli (FPC) were chosen for study since their predisposition to the tumour may be due to an inherited "initiation" event which is present in every cell. Thus, it might be predicted that skin fibroblasts from these patients would exhibit increased susceptibility to in vitro transformation by tumour promoters alone. In the case of skin fibroblasts from RB patients, transformation as assessed by the ability of the cells to grow in semi-solid medium and their migration in collagen gels did not occur. However, experiments involving skin fibroblasts from FPC patients showed certain of these cells to grow in semi-solid medium following treatment with the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) alone, although the pattern of migration of the parent cell population in collagen gels was unchanged and they were non-tumorigenic in nude mice. The clones which grew in semi-solid medium, although stable with regard to anchor-age-independent growth, were also unaltered in terms of their migration pattern in collagen gels and their tumorigenicity in nude mice, and were considered not to be completely transformed. Parallel cytogenetic analysis showed that, during the course of these transformation studies, TPA significantly increased not only tetraploidy but also the chromosome aberration frequency. Several quadriradial figures were noted.
dc.language.isoenen
dc.subject.meshAdult
dc.subject.meshAnimals
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshCell Movement
dc.subject.meshCell Transformation, Neoplastic
dc.subject.meshCells, Cultured
dc.subject.meshChild
dc.subject.meshChild, Preschool
dc.subject.meshColonic Polyps
dc.subject.meshEye Neoplasms
dc.subject.meshFemale
dc.subject.meshFetus
dc.subject.meshFibroblasts
dc.subject.meshHumans
dc.subject.meshInfant
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Nude
dc.subject.meshMiddle Aged
dc.subject.meshNeoplasm Transplantation
dc.subject.meshPhorbols
dc.subject.meshPregnancy
dc.subject.meshRetinoblastoma
dc.subject.meshSkin
dc.subject.meshTetradecanoylphorbol Acetate
dc.subject.meshTransplantation, Heterologous
dc.titleSusceptibility of skin fibroblasts from individuals genetically predisposed to cancer, to transformation by the tumour promoter 12-O-tetradecanoylphorbol-13-acetate.en
dc.typeArticleen
dc.identifier.eissn1097-0215
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Wilmslow Road, Withington, Manchester M20 9BX, UKen
dc.identifier.journalInternational Journal of Canceren
html.description.abstractSkin fibroblasts from patients with hereditary retinoblastoma (RB) and familial polyposis coli (FPC) were chosen for study since their predisposition to the tumour may be due to an inherited "initiation" event which is present in every cell. Thus, it might be predicted that skin fibroblasts from these patients would exhibit increased susceptibility to in vitro transformation by tumour promoters alone. In the case of skin fibroblasts from RB patients, transformation as assessed by the ability of the cells to grow in semi-solid medium and their migration in collagen gels did not occur. However, experiments involving skin fibroblasts from FPC patients showed certain of these cells to grow in semi-solid medium following treatment with the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) alone, although the pattern of migration of the parent cell population in collagen gels was unchanged and they were non-tumorigenic in nude mice. The clones which grew in semi-solid medium, although stable with regard to anchor-age-independent growth, were also unaltered in terms of their migration pattern in collagen gels and their tumorigenicity in nude mice, and were considered not to be completely transformed. Parallel cytogenetic analysis showed that, during the course of these transformation studies, TPA significantly increased not only tetraploidy but also the chromosome aberration frequency. Several quadriradial figures were noted.


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