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dc.contributor.authorFord, W L
dc.contributor.authorAllen, Terence D
dc.contributor.authorPitt, M A
dc.contributor.authorSmith, M E
dc.contributor.authorStoddart, R W
dc.date.accessioned2011-03-13T00:05:56Z
dc.date.available2011-03-13T00:05:56Z
dc.date.issued1984-07
dc.identifier.citationThe migration of lymphocytes across specialized vascular endothelium: VIII. Physical and chemical conditions influencing the surface morphology of lymphocytes and their ability to enter lymph nodes. 1984, 170 (3):377-90 Am J Anaten
dc.identifier.issn0002-9106
dc.identifier.pmid6383005
dc.identifier.doi10.1002/aja.1001700312
dc.identifier.urihttp://hdl.handle.net/10541/124427
dc.description.abstractThe introductory review amplifies the finding that simply holding lymphocytes in vitro reversibly compromises their ability to enter lymph nodes from the blood, although entry into the spleen is unaffected. The differential migration of T and B lymphocytes from the blood, lymphocyte traffic in athymic rats, and the secretion of a sulphated glycoconjugate by high endothelial cells in lymph nodes are also discussed. Original data are presented concerning the effects of varying the conditions under which lymphocytes are held in vitro (time, temperature, medium, centrifugation) on their ability to enter lymph nodes and also on their surface morphology. In general, conditions that reduced the number of microvilli and induced surface blebbing also tended to affect the delicate function of crossing specialized vascular endothelium; but there was no simple relationship between morphology and migratory behavior. The localization of lymphocytes to the bone marrow was augmented by holding them in vitro, and this effect was greater after holding at room temperature (RT) than at 0 degree C, in contrast to impaired entry into lymph nodes. Small amounts of heparin (10 units) injected along with lymphocytes significantly reduced early localization in lymph nodes. These findings have practical implications for the design of lymphocyte traffic experiments and are relevant to the mechanism of lymphocyte attachment to vascular endothelium, since the well-known effect of trypsinizing lymphocytes can be reproduced by maintenance in vitro.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshB-Lymphocytes
dc.subject.meshCell Movement
dc.subject.meshChemical Phenomena
dc.subject.meshChemistry
dc.subject.meshCytological Techniques
dc.subject.meshEndothelium
dc.subject.meshHeparin
dc.subject.meshLymph Nodes
dc.subject.meshLymphatic System
dc.subject.meshLymphocytes
dc.subject.meshRats
dc.subject.meshRats, Inbred Strains
dc.subject.meshSurface Properties
dc.subject.meshT-Lymphocytes
dc.subject.meshTemperature
dc.subject.meshThoracic Duct
dc.subject.meshVeins
dc.subject.meshVenules
dc.titleThe migration of lymphocytes across specialized vascular endothelium: VIII. Physical and chemical conditions influencing the surface morphology of lymphocytes and their ability to enter lymph nodes.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, England.en
dc.identifier.journalThe American Journal of Anatomyen
html.description.abstractThe introductory review amplifies the finding that simply holding lymphocytes in vitro reversibly compromises their ability to enter lymph nodes from the blood, although entry into the spleen is unaffected. The differential migration of T and B lymphocytes from the blood, lymphocyte traffic in athymic rats, and the secretion of a sulphated glycoconjugate by high endothelial cells in lymph nodes are also discussed. Original data are presented concerning the effects of varying the conditions under which lymphocytes are held in vitro (time, temperature, medium, centrifugation) on their ability to enter lymph nodes and also on their surface morphology. In general, conditions that reduced the number of microvilli and induced surface blebbing also tended to affect the delicate function of crossing specialized vascular endothelium; but there was no simple relationship between morphology and migratory behavior. The localization of lymphocytes to the bone marrow was augmented by holding them in vitro, and this effect was greater after holding at room temperature (RT) than at 0 degree C, in contrast to impaired entry into lymph nodes. Small amounts of heparin (10 units) injected along with lymphocytes significantly reduced early localization in lymph nodes. These findings have practical implications for the design of lymphocyte traffic experiments and are relevant to the mechanism of lymphocyte attachment to vascular endothelium, since the well-known effect of trypsinizing lymphocytes can be reproduced by maintenance in vitro.


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