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    The migration of lymphocytes across specialized vascular endothelium: VIII. Physical and chemical conditions influencing the surface morphology of lymphocytes and their ability to enter lymph nodes.

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    Authors
    Ford, W L
    Allen, Terence D
    Pitt, M A
    Smith, M E
    Stoddart, R W
    Affiliation
    Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, England.
    Issue Date
    1984-07
    
    Metadata
    Show full item record
    Abstract
    The introductory review amplifies the finding that simply holding lymphocytes in vitro reversibly compromises their ability to enter lymph nodes from the blood, although entry into the spleen is unaffected. The differential migration of T and B lymphocytes from the blood, lymphocyte traffic in athymic rats, and the secretion of a sulphated glycoconjugate by high endothelial cells in lymph nodes are also discussed. Original data are presented concerning the effects of varying the conditions under which lymphocytes are held in vitro (time, temperature, medium, centrifugation) on their ability to enter lymph nodes and also on their surface morphology. In general, conditions that reduced the number of microvilli and induced surface blebbing also tended to affect the delicate function of crossing specialized vascular endothelium; but there was no simple relationship between morphology and migratory behavior. The localization of lymphocytes to the bone marrow was augmented by holding them in vitro, and this effect was greater after holding at room temperature (RT) than at 0 degree C, in contrast to impaired entry into lymph nodes. Small amounts of heparin (10 units) injected along with lymphocytes significantly reduced early localization in lymph nodes. These findings have practical implications for the design of lymphocyte traffic experiments and are relevant to the mechanism of lymphocyte attachment to vascular endothelium, since the well-known effect of trypsinizing lymphocytes can be reproduced by maintenance in vitro.
    Citation
    The migration of lymphocytes across specialized vascular endothelium: VIII. Physical and chemical conditions influencing the surface morphology of lymphocytes and their ability to enter lymph nodes. 1984, 170 (3):377-90 Am J Anat
    Journal
    The American Journal of Anatomy
    URI
    http://hdl.handle.net/10541/124427
    DOI
    10.1002/aja.1001700312
    PubMed ID
    6383005
    Type
    Article
    Language
    en
    ISSN
    0002-9106
    ae974a485f413a2113503eed53cd6c53
    10.1002/aja.1001700312
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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