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    Rotational diffusion of calcium-dependent adenosine-5'-triphosphatase in sarcoplasmic reticulum: a detailed study.

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    Authors
    Restall, C J
    Dale, Robert E
    Murray, E K
    Gilbert, Charles W
    Chapman, D
    Affiliation
    Department of Biochemistry and Chemistry, Royal Free Hospital School of Medicine, London NW3 2PF
    Issue Date
    1984-12-18
    
    Metadata
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    Abstract
    The Ca2+-Mg2+ adenosine-5'-triphosphatase (ATPase) in sarcoplasmic reticulum has been covalently labeled with the phosphorescent triplet probe erythrosinyl 5-isothiocyanate. The rotational diffusion of the protein in the membrane at 25 degrees C was examined by measuring the time dependence of the phosphorescence emission anisotropy. Detailed analysis of both the total emission S(t) = Iv(t) + 2IH(t) and anisotropy R(t) = [Iv(t) - IH(t)]/[Iv(t) + 2IH(t)] curves shows the presence of multiple components. The latter is incompatible with a simple model of protein movement. The experimental data are consistent with a model in which the sum of four exponential components defines the phosphorescence decay. The anisotropy decay corresponds to a model in which the phosphor itself or a small phosphor-bearing segment reorients on a sub-microsecond time scale about an axis attached to a larger segment, which in turn reorients on a time scale of a few microseconds about an axis fixed in the frame of the ATPase. A fraction of the protein molecules rotate on a time scale of 100-200 microseconds about the normal to the bilayer, while the rest are rotationally stationary, at least on a sub-millisecond time scale.
    Citation
    Rotational diffusion of calcium-dependent adenosine-5'-triphosphatase in sarcoplasmic reticulum: a detailed study. 1984, 23 (26):6765-76 Biochemistry
    Journal
    Biochemistry
    URI
    http://hdl.handle.net/10541/124425
    DOI
    10.1021/bi00321a075
    PubMed ID
    6152181
    Type
    Article
    Language
    en
    ISSN
    0006-2960
    EISSN
    1520-4995
    ae974a485f413a2113503eed53cd6c53
    10.1021/bi00321a075
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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