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dc.contributor.authorHampson, I N
dc.contributor.authorGallagher, John T
dc.date.accessioned2011-03-13T00:26:17Z
dc.date.available2011-03-13T00:26:17Z
dc.date.issued1984-08-01
dc.identifier.citationSeparation of radiolabelled glycosaminoglycan oligosaccharides by polyacrylamide-gel electrophoresis. 1984, 221 (3):697-705 Biochem Jen
dc.identifier.issn0264-6021
dc.identifier.pmid6477495
dc.identifier.urihttp://hdl.handle.net/10541/124398
dc.description.abstractGlycosaminoglycan oligosaccharides generated by treatment of biosynthetically radiolabelled dermatan sulphate and hyaluronic acid with chondroitin AC lyase or testicular hyaluronidase may be resolved into a series of discrete bands by polyacrylamide-gel electrophoresis. Bands were identified by fixation in glacial acetic acid containing 20% (w/v) 2,5-diphenyloxazole followed by fluorography. The bands represented glycans which differed in size by one disaccharide unit. For the larger oligosaccharides (decasaccharides and above) of similar charge: mass ratio, there was a linear relationship between electrophoretic mobility and log Mr. However, the smaller species showed anomalous migration patterns. Consideration of the structures of the fragments produced by the different enzyme treatments suggests that copolymeric and homopolymeric oligosaccharides may be separated by polyacrylamide-gel electrophoresis. There are many potential applications of this technique, foremost amongst them being studies on the molecular size heterogeneity and patterns of enzyme-mediated depolymerization of native glycosaminoglycan chains and investigations into rates of polymer chain elongation and post-polymerization modification reactions so essential to glycosaminoglycan function.
dc.language.isoenen
dc.subject.meshChromatography, Gel
dc.subject.meshDensitometry
dc.subject.meshDermatan Sulfate
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshGlycosaminoglycans
dc.subject.meshHumans
dc.subject.meshHyaluronic Acid
dc.subject.meshLasers
dc.subject.meshOligosaccharides
dc.subject.meshTritium
dc.titleSeparation of radiolabelled glycosaminoglycan oligosaccharides by polyacrylamide-gel electrophoresis.en
dc.typeArticleen
dc.identifier.eissn1470-8728
dc.identifier.journalThe Biochemical Journalen
html.description.abstractGlycosaminoglycan oligosaccharides generated by treatment of biosynthetically radiolabelled dermatan sulphate and hyaluronic acid with chondroitin AC lyase or testicular hyaluronidase may be resolved into a series of discrete bands by polyacrylamide-gel electrophoresis. Bands were identified by fixation in glacial acetic acid containing 20% (w/v) 2,5-diphenyloxazole followed by fluorography. The bands represented glycans which differed in size by one disaccharide unit. For the larger oligosaccharides (decasaccharides and above) of similar charge: mass ratio, there was a linear relationship between electrophoretic mobility and log Mr. However, the smaller species showed anomalous migration patterns. Consideration of the structures of the fragments produced by the different enzyme treatments suggests that copolymeric and homopolymeric oligosaccharides may be separated by polyacrylamide-gel electrophoresis. There are many potential applications of this technique, foremost amongst them being studies on the molecular size heterogeneity and patterns of enzyme-mediated depolymerization of native glycosaminoglycan chains and investigations into rates of polymer chain elongation and post-polymerization modification reactions so essential to glycosaminoglycan function.


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