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    Separation of radiolabelled glycosaminoglycan oligosaccharides by polyacrylamide-gel electrophoresis.

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    Authors
    Hampson, I N
    Gallagher, John T
    Issue Date
    1984-08-01
    
    Metadata
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    Abstract
    Glycosaminoglycan oligosaccharides generated by treatment of biosynthetically radiolabelled dermatan sulphate and hyaluronic acid with chondroitin AC lyase or testicular hyaluronidase may be resolved into a series of discrete bands by polyacrylamide-gel electrophoresis. Bands were identified by fixation in glacial acetic acid containing 20% (w/v) 2,5-diphenyloxazole followed by fluorography. The bands represented glycans which differed in size by one disaccharide unit. For the larger oligosaccharides (decasaccharides and above) of similar charge: mass ratio, there was a linear relationship between electrophoretic mobility and log Mr. However, the smaller species showed anomalous migration patterns. Consideration of the structures of the fragments produced by the different enzyme treatments suggests that copolymeric and homopolymeric oligosaccharides may be separated by polyacrylamide-gel electrophoresis. There are many potential applications of this technique, foremost amongst them being studies on the molecular size heterogeneity and patterns of enzyme-mediated depolymerization of native glycosaminoglycan chains and investigations into rates of polymer chain elongation and post-polymerization modification reactions so essential to glycosaminoglycan function.
    Citation
    Separation of radiolabelled glycosaminoglycan oligosaccharides by polyacrylamide-gel electrophoresis. 1984, 221 (3):697-705 Biochem J
    Journal
    The Biochemical Journal
    URI
    http://hdl.handle.net/10541/124398
    PubMed ID
    6477495
    Type
    Article
    Language
    en
    ISSN
    0264-6021
    EISSN
    1470-8728
    Collections
    All Paterson Institute for Cancer Research

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