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dc.contributor.authorLord, Brian I
dc.contributor.authorHendry, Jolyon H
dc.contributor.authorKeene, J P
dc.contributor.authorHodgson, B W
dc.contributor.authorXu, C
dc.contributor.authorRezvani, M
dc.contributor.authorJordan, Thomas J
dc.date.accessioned2011-03-13T00:08:05Z
dc.date.available2011-03-13T00:08:05Z
dc.date.issued1984-07
dc.identifier.citationA comparison of low and high dose-rate radiation for recipient mice in spleen-colony studies. 1984, 17 (4):323-34 Cell Tissue Kineten
dc.identifier.issn0008-8730
dc.identifier.pmid6375870
dc.identifier.urihttp://hdl.handle.net/10541/124394
dc.description.abstractOver the last 15 years, endogenous spleen-colony formation in our mice, following lethal irradiation, has increased to an unacceptable level. It has been found necessary, therefore, to introduce a new method of preparing recipient mice for spleen-colony studies. Irradiation with low dose-rate 60Cobalt gamma rays has been compared with high dose-rate linear accelerator electrons, and their effects on endogenous spleen colony formation compared with earlier X and gamma ray dose-response data. It was found that a large dose (13.5 Gy) of gamma rays results in fewer endogenous colonies than 8.5 Gy of electrons, yet because of its low dose rate (14.1 X 10(-3) Gy/min) it has a marked sparing of the intestinal tissue as measured by the intestinal microcolony technique. This in turn permits better survival and, therefore, a 'healthier' animal for spleen-colony work. Exogenous colony formation is also lower in the low dose-rate, gamma-irradiated recipients and this is shown to be due to a reduced spleen-seeding efficiency. It is concluded that very low dose-rate radiation is preferable for haemopoietic ablation, that a mouse colony requires constant monitoring for changes of endogenous spleen-colony formation and that the spleen-seeding efficiency of CFU-s depends on the irradiation technique used--there is no absolute value for a given strain of mouse.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBone Marrow Transplantation
dc.subject.meshCell Count
dc.subject.meshCobalt Radioisotopes
dc.subject.meshDose-Response Relationship, Radiation
dc.subject.meshFemale
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Inbred C57BL
dc.subject.meshMice, Inbred DBA
dc.subject.meshSpleen
dc.titleA comparison of low and high dose-rate radiation for recipient mice in spleen-colony studies.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories and Radiotherapy Department, CHristie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalCell and Tissue Kineticsen
html.description.abstractOver the last 15 years, endogenous spleen-colony formation in our mice, following lethal irradiation, has increased to an unacceptable level. It has been found necessary, therefore, to introduce a new method of preparing recipient mice for spleen-colony studies. Irradiation with low dose-rate 60Cobalt gamma rays has been compared with high dose-rate linear accelerator electrons, and their effects on endogenous spleen colony formation compared with earlier X and gamma ray dose-response data. It was found that a large dose (13.5 Gy) of gamma rays results in fewer endogenous colonies than 8.5 Gy of electrons, yet because of its low dose rate (14.1 X 10(-3) Gy/min) it has a marked sparing of the intestinal tissue as measured by the intestinal microcolony technique. This in turn permits better survival and, therefore, a 'healthier' animal for spleen-colony work. Exogenous colony formation is also lower in the low dose-rate, gamma-irradiated recipients and this is shown to be due to a reduced spleen-seeding efficiency. It is concluded that very low dose-rate radiation is preferable for haemopoietic ablation, that a mouse colony requires constant monitoring for changes of endogenous spleen-colony formation and that the spleen-seeding efficiency of CFU-s depends on the irradiation technique used--there is no absolute value for a given strain of mouse.


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