• Login
    View Item 
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    A comparison of low and high dose-rate radiation for recipient mice in spleen-colony studies.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Lord, Brian I
    Hendry, Jolyon H
    Keene, J P
    Hodgson, B W
    Xu, C
    Rezvani, M
    Jordan, Thomas J
    Affiliation
    Paterson Laboratories and Radiotherapy Department, CHristie Hospital and Holt Radium Institute, Manchester M20 9BX
    Issue Date
    1984-07
    
    Metadata
    Show full item record
    Abstract
    Over the last 15 years, endogenous spleen-colony formation in our mice, following lethal irradiation, has increased to an unacceptable level. It has been found necessary, therefore, to introduce a new method of preparing recipient mice for spleen-colony studies. Irradiation with low dose-rate 60Cobalt gamma rays has been compared with high dose-rate linear accelerator electrons, and their effects on endogenous spleen colony formation compared with earlier X and gamma ray dose-response data. It was found that a large dose (13.5 Gy) of gamma rays results in fewer endogenous colonies than 8.5 Gy of electrons, yet because of its low dose rate (14.1 X 10(-3) Gy/min) it has a marked sparing of the intestinal tissue as measured by the intestinal microcolony technique. This in turn permits better survival and, therefore, a 'healthier' animal for spleen-colony work. Exogenous colony formation is also lower in the low dose-rate, gamma-irradiated recipients and this is shown to be due to a reduced spleen-seeding efficiency. It is concluded that very low dose-rate radiation is preferable for haemopoietic ablation, that a mouse colony requires constant monitoring for changes of endogenous spleen-colony formation and that the spleen-seeding efficiency of CFU-s depends on the irradiation technique used--there is no absolute value for a given strain of mouse.
    Citation
    A comparison of low and high dose-rate radiation for recipient mice in spleen-colony studies. 1984, 17 (4):323-34 Cell Tissue Kinet
    Journal
    Cell and Tissue Kinetics
    URI
    http://hdl.handle.net/10541/124394
    PubMed ID
    6375870
    Type
    Article
    Language
    en
    ISSN
    0008-8730
    Collections
    All Christie Publications

    entitlement

    Related articles

    • Murine haemopoietic stem cells with long-term engraftment and marrow repopulating ability are more resistant to gamma-radiation than are spleen colony forming cells.
    • Authors: Ploemacher RE, van Os R, van Beurden CA, Down JD
    • Issue date: 1992 Apr
    • [The enhanced radioresistance (adaptive response) in vivo of splenic colony-forming units (CFU-S) following the exposure of mice to 60Co gamma rays at low doses].
    • Authors: Saenko AS, Semenets TN, Semina OV
    • Issue date: 1991 Sep-Oct
    • Increase in endogenous spleen colonies without recovery of blood cell counts in radioadaptive survival response in C57BL/6 mice.
    • Authors: Yonezawa M, Horie K, Kondo H, Kubo K
    • Issue date: 2004 Feb
    • Survival of spleen colony-forming units (CFU-S) of irradiated bone marrow cells in mice: evidence for the existence of a radioresistant subfraction.
    • Authors: Inoue T, Hirabayashi Y, Mitsui H, Sasaki H, Cronkite EP, Bullis JE Jr, Bond VP, Yoshida K
    • Issue date: 1995 Nov
    • [The radiosensitivity of hematopoietic stem cells from mice forming splenic colonies after 8 and 12 days following bone marrow cell transplantation (CFU-S-8 and CFU-S-12)].
    • Authors: Trishkina AI, Konopliannikov AG
    • Issue date: 1992 Mar-Apr
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.