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The effect of vinca alkaloids in enhancing the sensitivity of a methotrexate-resistant (L1210/R7A) line, studied by flow cytometric and chromosome number analysis.

dc.contributor.authorMcGown, Alan T
dc.contributor.authorPoppitt, David G
dc.contributor.authorSwindell, Ric
dc.contributor.authorFox, Brian W
dc.date.accessioned2011-03-12T23:55:49Z
dc.date.available2011-03-12T23:55:49Z
dc.date.issued1984
dc.identifier.citationThe effect of vinca alkaloids in enhancing the sensitivity of a methotrexate-resistant (L1210/R7A) line, studied by flow cytometric and chromosome number analysis. 1984, 13 (1):47-53 Cancer Chemother. Pharmacolen
dc.identifier.issn0344-5704
dc.identifier.pmid6733842
dc.identifier.doi10.1007/BF00401447
dc.identifier.urihttp://hdl.handle.net/10541/124393
dc.description.abstractTwo L1210 murine lymphoma cell lines sensitive and resistant to methotrexate (L1210 and L1210/ R7A , respectively) and previously shown to exhibit collateral sensitivity to the vinca alkaloids have been studied by flow cytofluorimetric techniques following propidium iodide staining of the DNA. Following treatment with a range of concentrations of vincristine, both cell lines showed a build-up of fluorescence in the 4n position. However, the methotrexate-resistant cell line exhibited this effect at lower doses of vincristine. On an equimolar basis, the vinca alkaloids ranked for intensity of this effect in the order vinblastine greater than vindesine greater than vincristine. DNA fluorescent histograms following various times of continuous exposure to vincristine showed an accumulation of material at the 8n position, which was shown by chromosome analysis to be due to polyploidy. It was concluded that methotrexate-resistant cells (L1210/ R7A ) experience difficulty in traversing mitosis and this difficulty is enhanced by the vinca alkaloids.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshChromosomes
dc.subject.meshDNA, Neoplasm
dc.subject.meshDrug Resistance
dc.subject.meshFlow Cytometry
dc.subject.meshLeukemia L1210
dc.subject.meshMethotrexate
dc.subject.meshMice
dc.subject.meshPolyploidy
dc.subject.meshVinblastine
dc.subject.meshVinca Alkaloids
dc.subject.meshVincristine
dc.subject.meshVindesine
dc.titleThe effect of vinca alkaloids in enhancing the sensitivity of a methotrexate-resistant (L1210/R7A) line, studied by flow cytometric and chromosome number analysis.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories and Radiotherapy Department, CHristie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalCancer Chemotherapy and Pharmacologyen
html.description.abstractTwo L1210 murine lymphoma cell lines sensitive and resistant to methotrexate (L1210 and L1210/ R7A , respectively) and previously shown to exhibit collateral sensitivity to the vinca alkaloids have been studied by flow cytofluorimetric techniques following propidium iodide staining of the DNA. Following treatment with a range of concentrations of vincristine, both cell lines showed a build-up of fluorescence in the 4n position. However, the methotrexate-resistant cell line exhibited this effect at lower doses of vincristine. On an equimolar basis, the vinca alkaloids ranked for intensity of this effect in the order vinblastine greater than vindesine greater than vincristine. DNA fluorescent histograms following various times of continuous exposure to vincristine showed an accumulation of material at the 8n position, which was shown by chromosome analysis to be due to polyploidy. It was concluded that methotrexate-resistant cells (L1210/ R7A ) experience difficulty in traversing mitosis and this difficulty is enhanced by the vinca alkaloids.


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