Show simple item record

dc.contributor.authorSchor, Ana M
dc.contributor.authorSchor, Seth L
dc.contributor.authorAllen, Terence D
dc.date.accessioned2011-03-12T23:53:50Z
dc.date.available2011-03-12T23:53:50Z
dc.date.issued1984
dc.identifier.citationThe synthesis of subendothelial matrix by bovine aortic endothelial cells in culture. 1984, 16 (5):677-91 Tissue Cellen
dc.identifier.issn0040-8166
dc.identifier.pmid6569762
dc.identifier.doi10.1016/0040-8166(84)90002-8
dc.identifier.urihttp://hdl.handle.net/10541/124392
dc.description.abstractBovine aortic endothelial cells cultured on collagenous or plastic substrata continuously synthesize and deposit a subendothelial matrix, independently of whether the cells are in the logarithmic or the stationary phase of growth. This subendothelial matrix contains fibrillar and amorphous elements comparable with those observed in the subendothelium in vivo. Deposition of subendothelial matrix on a collagen gel substratum both started earlier and progressed at approximately double the rate than that on denatured collagen. The relative composition of the subendothelial matrix was assessed by sequential incubation with trypsin, elastase and collagenase (Jones et al., 1979). The subendothelial matrix deposited on collagen gels by early confluent cultures and late post-confluent cultures differed in their enzyme sensitivity. These age-related changes in the enzyme sensitivity of the subendothelial matrix were characteristic for each cloned cell population examined. Comparable variations in the composition of the subendothelial matrix were not observed when the cells were cultured on plastic or gelatin-coated dishes; the subendothelial matrix deposited on these two substrata contained considerably more trypsin-sensitive material and less elastase and collagenase-sensitive material than the matrix deposited on native collagen gels. Age-related changes in the enzyme sensitivity of the subendothelial matrix deposited on collagen gels was found to be a function of the time elapsed since confluence and it was not related to the time elapsed since plating or to the number of cells present.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAorta
dc.subject.meshAscorbic Acid
dc.subject.meshCattle
dc.subject.meshCollagen
dc.subject.meshCulture Media
dc.subject.meshEndothelium
dc.subject.meshExtracellular Matrix
dc.subject.meshMicroscopy, Electron
dc.subject.meshPancreatic Elastase
dc.subject.meshTrypsin
dc.titleThe synthesis of subendothelial matrix by bovine aortic endothelial cells in culture.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Medical Oncology, Christie Hospital and Holt Radium Institute, Manchester, M20 9BX, UK.en
dc.identifier.journalTissue & Cellen
html.description.abstractBovine aortic endothelial cells cultured on collagenous or plastic substrata continuously synthesize and deposit a subendothelial matrix, independently of whether the cells are in the logarithmic or the stationary phase of growth. This subendothelial matrix contains fibrillar and amorphous elements comparable with those observed in the subendothelium in vivo. Deposition of subendothelial matrix on a collagen gel substratum both started earlier and progressed at approximately double the rate than that on denatured collagen. The relative composition of the subendothelial matrix was assessed by sequential incubation with trypsin, elastase and collagenase (Jones et al., 1979). The subendothelial matrix deposited on collagen gels by early confluent cultures and late post-confluent cultures differed in their enzyme sensitivity. These age-related changes in the enzyme sensitivity of the subendothelial matrix were characteristic for each cloned cell population examined. Comparable variations in the composition of the subendothelial matrix were not observed when the cells were cultured on plastic or gelatin-coated dishes; the subendothelial matrix deposited on these two substrata contained considerably more trypsin-sensitive material and less elastase and collagenase-sensitive material than the matrix deposited on native collagen gels. Age-related changes in the enzyme sensitivity of the subendothelial matrix deposited on collagen gels was found to be a function of the time elapsed since confluence and it was not related to the time elapsed since plating or to the number of cells present.


Files in this item

This item appears in the following Collection(s)

Show simple item record