The synthesis of subendothelial matrix by bovine aortic endothelial cells in culture.
Affiliation
CRC Department of Medical Oncology, Christie Hospital and Holt Radium Institute, Manchester, M20 9BX, UK.Issue Date
1984
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Show full item recordAbstract
Bovine aortic endothelial cells cultured on collagenous or plastic substrata continuously synthesize and deposit a subendothelial matrix, independently of whether the cells are in the logarithmic or the stationary phase of growth. This subendothelial matrix contains fibrillar and amorphous elements comparable with those observed in the subendothelium in vivo. Deposition of subendothelial matrix on a collagen gel substratum both started earlier and progressed at approximately double the rate than that on denatured collagen. The relative composition of the subendothelial matrix was assessed by sequential incubation with trypsin, elastase and collagenase (Jones et al., 1979). The subendothelial matrix deposited on collagen gels by early confluent cultures and late post-confluent cultures differed in their enzyme sensitivity. These age-related changes in the enzyme sensitivity of the subendothelial matrix were characteristic for each cloned cell population examined. Comparable variations in the composition of the subendothelial matrix were not observed when the cells were cultured on plastic or gelatin-coated dishes; the subendothelial matrix deposited on these two substrata contained considerably more trypsin-sensitive material and less elastase and collagenase-sensitive material than the matrix deposited on native collagen gels. Age-related changes in the enzyme sensitivity of the subendothelial matrix deposited on collagen gels was found to be a function of the time elapsed since confluence and it was not related to the time elapsed since plating or to the number of cells present.Citation
The synthesis of subendothelial matrix by bovine aortic endothelial cells in culture. 1984, 16 (5):677-91 Tissue CellJournal
Tissue & CellDOI
10.1016/0040-8166(84)90002-8PubMed ID
6569762Type
ArticleLanguage
enISSN
0040-8166ae974a485f413a2113503eed53cd6c53
10.1016/0040-8166(84)90002-8