Show simple item record

dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorMoore, James Ven
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2011-03-06T22:38:17Z
dc.date.available2011-03-06T22:38:17Z
dc.date.issued1984-01
dc.identifier.citationThe proliferative status of microcolony-forming cells in mouse small intestine. 1984, 17 (1):41-7 Cell Tissue Kineten
dc.identifier.issn0008-8730
dc.identifier.pmid6362884
dc.identifier.urihttp://hdl.handle.net/10541/123696
dc.description.abstractThe technique of thymidine (TdR) suicide has been used with the intestinal microcolony assay to demonstrate that in the middle of the light cycle, nearly all intestinal clonogenic cells, in the B6D2F1 mice used in these experiments, were not in S phase. Doses of tritiated thymidine [3H]TdR up to 1 mCi/mouse did not kill a significant fraction of those clonogenic cells which survived a test dose of 12 Gy gamma-rays. This finding supports some data in the literature, but conflicts with others. However, the suicide technique was found in the studies reported here to be very efficient in sterilizing clonogenic cells in the middle of the dark cycle, and also in a regenerating epithelium at day 3 after a dose of 9 Gy. This implies that the technique can discriminate well between populations of clonogenic cells which differ in their content of cells in S phase. The lack of a suicide effect in the middle of the light cycle indicates that the majority of proliferative epithelial cells are not clonogenic.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Division
dc.subject.meshCell Survival
dc.subject.meshCircadian Rhythm
dc.subject.meshDose-Response Relationship, Radiation
dc.subject.meshFemale
dc.subject.meshGamma Rays
dc.subject.meshInterphase
dc.subject.meshIntestine, Small
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshStem Cells
dc.subject.meshWhole-Body Irradiation
dc.titleThe proliferative status of microcolony-forming cells in mouse small intestine.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalCell and Tissue Kineticsen
html.description.abstractThe technique of thymidine (TdR) suicide has been used with the intestinal microcolony assay to demonstrate that in the middle of the light cycle, nearly all intestinal clonogenic cells, in the B6D2F1 mice used in these experiments, were not in S phase. Doses of tritiated thymidine [3H]TdR up to 1 mCi/mouse did not kill a significant fraction of those clonogenic cells which survived a test dose of 12 Gy gamma-rays. This finding supports some data in the literature, but conflicts with others. However, the suicide technique was found in the studies reported here to be very efficient in sterilizing clonogenic cells in the middle of the dark cycle, and also in a regenerating epithelium at day 3 after a dose of 9 Gy. This implies that the technique can discriminate well between populations of clonogenic cells which differ in their content of cells in S phase. The lack of a suicide effect in the middle of the light cycle indicates that the majority of proliferative epithelial cells are not clonogenic.


This item appears in the following Collection(s)

Show simple item record