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dc.contributor.authorDodd, Nicholas J F
dc.contributor.authorSwartz, H M
dc.date.accessioned2011-03-06T22:28:38Z
dc.date.available2011-03-06T22:28:38Z
dc.date.issued1984-01
dc.identifier.citationThe nature of the ESR signal in lyophilized tissue and its relevance to malignancy. 1984, 49 (1):65-71 Br. J. Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid6318790
dc.identifier.urihttp://hdl.handle.net/10541/123690
dc.description.abstractComparison of 9 and 35 GHz spectra, obtained from frozen and lyophilized tissues, with those from model systems containing ascorbic acid, confirm that the major component of the "lyophilization signal" of tissue is the ascorbyl radical, stabilized by adsorption on an inert matrix. The magnitude of the signal under anoxic conditions is shown to be a measure of cellular damage, which allows intracellular ascorbic acid to be oxidized. On exposure of lyophilized samples to air, the signal increases due to autoxidation of the available tissue ascorbic acid. Under moist atmospheric conditions the ascorbyl radicals readily decay, leaving other radicals, which appear to be formed by interaction of ascorbic acid or ascorbyl radicals with some tissue component. The results show that, although widely studied, the free radical ESR signal of lyophilized tissue is not unique to tumour and has no relevance to malignancy.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAscorbic Acid
dc.subject.meshElectron Spin Resonance Spectroscopy
dc.subject.meshFree Radicals
dc.subject.meshFreeze Drying
dc.subject.meshMale
dc.subject.meshMuscles
dc.subject.meshNeoplasms
dc.subject.meshRats
dc.subject.meshRats, Inbred Strains
dc.titleThe nature of the ESR signal in lyophilized tissue and its relevance to malignancy.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, U.K.en
dc.identifier.journalBritish Journal of Canceren
html.description.abstractComparison of 9 and 35 GHz spectra, obtained from frozen and lyophilized tissues, with those from model systems containing ascorbic acid, confirm that the major component of the "lyophilization signal" of tissue is the ascorbyl radical, stabilized by adsorption on an inert matrix. The magnitude of the signal under anoxic conditions is shown to be a measure of cellular damage, which allows intracellular ascorbic acid to be oxidized. On exposure of lyophilized samples to air, the signal increases due to autoxidation of the available tissue ascorbic acid. Under moist atmospheric conditions the ascorbyl radicals readily decay, leaving other radicals, which appear to be formed by interaction of ascorbic acid or ascorbyl radicals with some tissue component. The results show that, although widely studied, the free radical ESR signal of lyophilized tissue is not unique to tumour and has no relevance to malignancy.


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