The nature of the ESR signal in lyophilized tissue and its relevance to malignancy.
AffiliationPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, U.K.
MetadataShow full item record
AbstractComparison of 9 and 35 GHz spectra, obtained from frozen and lyophilized tissues, with those from model systems containing ascorbic acid, confirm that the major component of the "lyophilization signal" of tissue is the ascorbyl radical, stabilized by adsorption on an inert matrix. The magnitude of the signal under anoxic conditions is shown to be a measure of cellular damage, which allows intracellular ascorbic acid to be oxidized. On exposure of lyophilized samples to air, the signal increases due to autoxidation of the available tissue ascorbic acid. Under moist atmospheric conditions the ascorbyl radicals readily decay, leaving other radicals, which appear to be formed by interaction of ascorbic acid or ascorbyl radicals with some tissue component. The results show that, although widely studied, the free radical ESR signal of lyophilized tissue is not unique to tumour and has no relevance to malignancy.
CitationThe nature of the ESR signal in lyophilized tissue and its relevance to malignancy. 1984, 49 (1):65-71 Br. J. Cancer
JournalBritish Journal of Cancer
- The significance of electron spin resonance of the ascorbic acid radical in freeze dried human brain tumours and oedematous or normal periphery.
- Authors: Mueller HW, Tannert S
- Issue date: 1986 Mar
- ESR investigations on lyophilized blood: mixtures with ascorbic acid.
- Authors: Neubacher H
- Issue date: 1984 Jan-Feb
- ESR signals of lyophilized tissue.
- Authors: Dodd NJ, Swartz HM
- Issue date: 1980 Aug
- Paramagnetic changes in cancer: DMBA-induced tumours studied in non-lyophilized and lyophilized tissues.
- Authors: Gutierrez PL, Swartz HM, Wilkinson EJ
- Issue date: 1979 Mar
- Paramagnetic changes in cancer: growth of Walker 256 carcinoma studied in frozen and lyophilized tissues.
- Authors: Gutierrez PL, Swartz HM
- Issue date: 1979 Jan