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dc.contributor.authorWhetton, Anthony D
dc.contributor.authorBazill, G W
dc.contributor.authorDexter, T Michael
dc.date.accessioned2011-03-06T22:48:59Z
dc.date.available2011-03-06T22:48:59Z
dc.date.issued1984-02
dc.identifier.citationHaemopoietic cell growth factor mediates cell survival via its action on glucose transport. 1984, 3 (2):409-13 EMBO J.en
dc.identifier.issn0261-4189
dc.identifier.pmid6370682
dc.identifier.urihttp://hdl.handle.net/10541/123673
dc.description.abstractA number of haematopoietic precursor cell lines have been established which exhibit an absolute dependence on haematopoietic cell growth factor (HCGF) which is secreted by WEHI-3 myelomonocytic leukaemia cells. In the presence of HCGF, ATP levels are maintained in these factor-dependent cells (FDC-P cells); in the absence of HCGF, intracellular ATP levels undergo a steady depletion. The cell death that follows this ATP depletion can be prevented by supplying exogenous ATP suggesting that HCGF maintains these cells via its effects on energy metabolism. We have investigated the effect of HCGF on FDC-P cells further and found that: (i) HCGF markedly and rapidly increases lactate production; (ii) high extracellular glucose or glycolytic intermediate concentrations can maintain FDC-P cell viability to some extent whilst stimulating lactate production; (iii) the uptake of 2-deoxyglucose by FDC-P2 cells is stimulated by HCGF in a dose-dependent fashion. This uptake is inhibited by cytochalasin B; (iv) HCGF does not stimulate L-glucose uptake by FDC-P cells. These results suggest that HCGF acts to maintain FDC-P cells via its action on glucose transport. The significance of these results to haemopoiesis and leukaemogenesis is discussed.
dc.language.isoenen
dc.subject.meshAdenosine Triphosphate
dc.subject.meshBiological Transport, Active
dc.subject.meshCell Survival
dc.subject.meshCytochalasin B
dc.subject.meshEnergy Metabolism
dc.subject.meshGlucose
dc.subject.meshGlycolysis
dc.subject.meshGrowth Substances
dc.subject.meshHematopoietic Cell Growth Factors
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHumans
dc.subject.meshLactates
dc.subject.meshLeukemia, Myeloid
dc.titleHaemopoietic cell growth factor mediates cell survival via its action on glucose transport.en
dc.typeArticleen
dc.contributor.departmentExperimental Haematology Section, Paterson Laboratories, Christie Hospital and Radium Institute, Withington, Manchester M20 9BX, UKen
dc.identifier.journalEMBO Journalen
html.description.abstractA number of haematopoietic precursor cell lines have been established which exhibit an absolute dependence on haematopoietic cell growth factor (HCGF) which is secreted by WEHI-3 myelomonocytic leukaemia cells. In the presence of HCGF, ATP levels are maintained in these factor-dependent cells (FDC-P cells); in the absence of HCGF, intracellular ATP levels undergo a steady depletion. The cell death that follows this ATP depletion can be prevented by supplying exogenous ATP suggesting that HCGF maintains these cells via its effects on energy metabolism. We have investigated the effect of HCGF on FDC-P cells further and found that: (i) HCGF markedly and rapidly increases lactate production; (ii) high extracellular glucose or glycolytic intermediate concentrations can maintain FDC-P cell viability to some extent whilst stimulating lactate production; (iii) the uptake of 2-deoxyglucose by FDC-P2 cells is stimulated by HCGF in a dose-dependent fashion. This uptake is inhibited by cytochalasin B; (iv) HCGF does not stimulate L-glucose uptake by FDC-P cells. These results suggest that HCGF acts to maintain FDC-P cells via its action on glucose transport. The significance of these results to haemopoiesis and leukaemogenesis is discussed.


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