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dc.contributor.authorBoettiger, David
dc.contributor.authorAnderson, Steven
dc.contributor.authorDexter, T Michael
dc.date.accessioned2011-03-06T22:42:41Z
dc.date.available2011-03-06T22:42:41Z
dc.date.issued1984-03
dc.identifier.citationEffect of src infection on long-term marrow cultures: increased self-renewal of hemopoietic progenitor cells without leukemia. 1984, 36 (3):763-73 Cellen
dc.identifier.issn0092-8674
dc.identifier.pmid6321038
dc.identifier.doi10.1016/0092-8674(84)90356-8
dc.identifier.urihttp://hdl.handle.net/10541/123671
dc.description.abstractLong-term marrow cultures prepared from mice have been infected with a molecular recombinant of Rous sarcoma virus and murine amphitropic leukemia virus. This resulted in introduction of the src gene into the cultured cells and expression of its protein kinase function. The infected cultures displayed an altered balance in the accumulation of cells in different compartments of granulocyte differentiation. There was a dramatic increase in the stem cell (CFU-S) compartment and the committed progenitor cell (GM-CFC) compartment and a decrease in mature granulocytes. The altered balance appears to be caused by intrinsic alterations in the CFU-S and GM-CFC themselves, which increase their "self-renewal" capacity at the expense of cell differentiation. Remarkably, unlike its effects in other systems, src did not produce a neoplastic transformation of the hemopoietic cells.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAvian Sarcoma Viruses
dc.subject.meshBone Marrow Cells
dc.subject.meshCell Adhesion
dc.subject.meshCell Differentiation
dc.subject.meshCell Transformation, Viral
dc.subject.meshColony-Forming Units Assay
dc.subject.meshGranulocytes
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshLeukemia Virus, Murine
dc.subject.meshMice
dc.subject.meshOncogenes
dc.titleEffect of src infection on long-term marrow cultures: increased self-renewal of hemopoietic progenitor cells without leukemia.en
dc.typeArticleen
dc.contributor.departmentDepartment of Microbiology University of Pennsylvania Philadelphia, Pennsylvania 19104en
dc.identifier.journalCellen
html.description.abstractLong-term marrow cultures prepared from mice have been infected with a molecular recombinant of Rous sarcoma virus and murine amphitropic leukemia virus. This resulted in introduction of the src gene into the cultured cells and expression of its protein kinase function. The infected cultures displayed an altered balance in the accumulation of cells in different compartments of granulocyte differentiation. There was a dramatic increase in the stem cell (CFU-S) compartment and the committed progenitor cell (GM-CFC) compartment and a decrease in mature granulocytes. The altered balance appears to be caused by intrinsic alterations in the CFU-S and GM-CFC themselves, which increase their "self-renewal" capacity at the expense of cell differentiation. Remarkably, unlike its effects in other systems, src did not produce a neoplastic transformation of the hemopoietic cells.


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