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dc.contributor.authorLomax, M
dc.contributor.authorBarnes, D
dc.contributor.authorGilchrist, R
dc.contributor.authorPicksley, S
dc.contributor.authorVarley, Jennifer
dc.contributor.authorCamplejohn, R
dc.date.accessioned2011-02-05T11:22:15Z
dc.date.available2011-02-05T11:22:15Z
dc.date.issued1997-04-17
dc.identifier.citationTwo functional assays employed to detect an unusual mutation in the oligomerisation domain of p53 in a Li-Fraumeni like family. 1997, 14 (15):1869-74 Oncogeneen
dc.identifier.issn0950-9232
dc.identifier.pmid9150393
dc.identifier.doi10.1038/sj.onc.1201133
dc.identifier.urihttp://hdl.handle.net/10541/121305
dc.description.abstractPrevious investigations of a Li - Fraumeni like family (Barnes et al., 1992) demonstrated that both the proband and her mother had elevated p53 protein levels in both tumour tissue and normal tissue at sites distant from the tumour, although no mutation was found in the p53 gene. In the present study two recently described functional assays for p53, an apoptotic assay and the functional assay for the separation of alleles in yeast (FASAY), have been employed to study the functional activity of p53 from this patient. The results of the apoptotic assay demonstrated that this patient had a p53 functional defect and the FASAY result suggested that this defect was in fact a germline mutation of the p53 gene. A point mutation of codon 337, which results in an amino acid substitution of a cysteine for an arginine, was demonstrated initially in cDNA and was confirmed by sequencing of genomic DNA. This is an unusual mutation as it is in the oligomerisation domain of p53, in contrast to the majority of p53 mutations which are in the core DNA binding domain. This mutation results in a protein which still retains partial transactivational activity in the FASAY. The mutation of codon 337 is only the second reported case of a germline missense mutation occurring in the oligomerisation domain of p53.
dc.language.isoenen
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshAlleles
dc.subject.meshApoptosis
dc.subject.meshCodon
dc.subject.meshDNA, Complementary
dc.subject.meshGenes, p53
dc.subject.meshGerm-Line Mutation
dc.subject.meshHumans
dc.subject.meshLi-Fraumeni Syndrome
dc.subject.meshLymphocytes
dc.subject.meshPoint Mutation
dc.subject.meshProtein Structure, Tertiary
dc.subject.meshTransformation, Genetic
dc.subject.meshTumor Suppressor Protein p53
dc.titleTwo functional assays employed to detect an unusual mutation in the oligomerisation domain of p53 in a Li-Fraumeni like family.en
dc.typeArticleen
dc.contributor.departmentRichard Dimbleby Department of Cancer Research, UMDS, St. Thomas' Hospital, London, UK.en
dc.identifier.journalOncogeneen
html.description.abstractPrevious investigations of a Li - Fraumeni like family (Barnes et al., 1992) demonstrated that both the proband and her mother had elevated p53 protein levels in both tumour tissue and normal tissue at sites distant from the tumour, although no mutation was found in the p53 gene. In the present study two recently described functional assays for p53, an apoptotic assay and the functional assay for the separation of alleles in yeast (FASAY), have been employed to study the functional activity of p53 from this patient. The results of the apoptotic assay demonstrated that this patient had a p53 functional defect and the FASAY result suggested that this defect was in fact a germline mutation of the p53 gene. A point mutation of codon 337, which results in an amino acid substitution of a cysteine for an arginine, was demonstrated initially in cDNA and was confirmed by sequencing of genomic DNA. This is an unusual mutation as it is in the oligomerisation domain of p53, in contrast to the majority of p53 mutations which are in the core DNA binding domain. This mutation results in a protein which still retains partial transactivational activity in the FASAY. The mutation of codon 337 is only the second reported case of a germline missense mutation occurring in the oligomerisation domain of p53.


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