Two functional assays employed to detect an unusual mutation in the oligomerisation domain of p53 in a Li-Fraumeni like family.
AffiliationRichard Dimbleby Department of Cancer Research, UMDS, St. Thomas' Hospital, London, UK.
MetadataShow full item record
AbstractPrevious investigations of a Li - Fraumeni like family (Barnes et al., 1992) demonstrated that both the proband and her mother had elevated p53 protein levels in both tumour tissue and normal tissue at sites distant from the tumour, although no mutation was found in the p53 gene. In the present study two recently described functional assays for p53, an apoptotic assay and the functional assay for the separation of alleles in yeast (FASAY), have been employed to study the functional activity of p53 from this patient. The results of the apoptotic assay demonstrated that this patient had a p53 functional defect and the FASAY result suggested that this defect was in fact a germline mutation of the p53 gene. A point mutation of codon 337, which results in an amino acid substitution of a cysteine for an arginine, was demonstrated initially in cDNA and was confirmed by sequencing of genomic DNA. This is an unusual mutation as it is in the oligomerisation domain of p53, in contrast to the majority of p53 mutations which are in the core DNA binding domain. This mutation results in a protein which still retains partial transactivational activity in the FASAY. The mutation of codon 337 is only the second reported case of a germline missense mutation occurring in the oligomerisation domain of p53.
CitationTwo functional assays employed to detect an unusual mutation in the oligomerisation domain of p53 in a Li-Fraumeni like family. 1997, 14 (15):1869-74 Oncogene
- A germline 2.35 kb deletion of p53 genomic DNA creating a specific loss of the oligomerization domain inherited in a Li-Fraumeni syndrome family.
- Authors: Plummer SJ, Santibáñez-Koref M, Kurosaki T, Liao S, Noble B, Fain PR, Anton-Culver H, Casey G
- Issue date: 1994 Nov
- Genetic and functional studies of a germline TP53 splicing mutation in a Li-Fraumeni-like family.
- Authors: Varley JM, Chapman P, McGown G, Thorncroft M, White GR, Greaves MJ, Scott D, Spreadborough A, Tricker KJ, Birch JM, Evans DG, Reddel R, Camplejohn RS, Burn J, Boyle JM
- Issue date: 1998 Jun 25
- Characterization of the oligomerization defects of two p53 mutants found in families with Li-Fraumeni and Li-Fraumeni-like syndrome.
- Authors: Davison TS, Yin P, Nie E, Kay C, Arrowsmith CH
- Issue date: 1998 Aug 6
- A previously undescribed mutation within the tetramerisation domain of TP53 in a family with Li-Fraumeni syndrome.
- Authors: Varley JM, McGown G, Thorncroft M, Cochrane S, Morrison P, Woll P, Kelsey AM, Mitchell EL, Boyle J, Birch JM, Evans DG
- Issue date: 1996 Jun 6
- Analysis of p53 status in human cell lines using a functional assay in yeast: detection of new non-sense p53 mutation in codon 124.
- Authors: Smardová J, Pavlová S, Svitáková M, Grochová D, Ravcuková B
- Issue date: 2005 Oct