SR-FTIR spectroscopy of renal epithelial carcinoma side population cells displaying stem cell-like characteristics.
Authors
Hughes, CLiew, M
Sachdeva, A
Bassan, P
Dumas, P
Hart, Claire A
Brown, Michael D
Clarke, Noel W
Gardner, Peter
Affiliation
Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK M1 7DN.Issue Date
2010-12
Metadata
Show full item recordAbstract
It is hypothesized that cells with stem cell-like properties may be influential in carcinogenesis, possessing the ability to self-renew, produce differentiated daughter cells and resist environmental or therapeutic injury. This has led to a surge in interest in identifying and characterizing the tumour initiating or cancer stem cell (CSC) with the aim of discovering novel diagnostic and prognostic markers and of understanding the basic biology with the ultimate aim of generating new therapeutic approaches and biomarkers. However, a major hurdle to this process has been the lack of a truly specific cancer stem cell biomarker allied to the rarity of these cells. This has led to problems in characterising these CSCs by traditional '-omic' techniques. Using a renal carcinoma cell line model, we show that synchrotron radiation-Fourier transform infrared (SR-FTIR) spectroscopy is a suitable tool to measure discrete differences in the biochemistry of small numbers of single-cells. Using the chemometric techniques of Principal Component and Linear Discriminant Analysis (PCA and LDA) for multivariate reduction, biochemical differences between the cells from different sub-populations were evaluated. Results found lipid and phosphodiester vibrations to be particularly good discriminating markers in the spectra of these stem-like cells, relative to the more differentiated, proliferating cells that make up the majority of the cell population.Citation
SR-FTIR spectroscopy of renal epithelial carcinoma side population cells displaying stem cell-like characteristics. 2010, 135 (12):3133-41 AnalystJournal
The AnalystDOI
10.1039/c0an00574fPubMed ID
20981365Type
ArticleLanguage
enISSN
1364-5528ae974a485f413a2113503eed53cd6c53
10.1039/c0an00574f
Scopus Count
Collections
Related articles
- Using Fourier transform infrared spectroscopy to evaluate biological effects induced by photodynamic therapy.
- Authors: Lima CA, Goulart VP, Correa L, Zezell DM
- Issue date: 2016 Jul
- Synchrotron-Based Fourier-Transform Infrared Micro-Spectroscopy (SR-FTIRM) Fingerprint of the Small Anionic Molecule Cobaltabis(dicarbollide) Uptake in Glioma Stem Cells.
- Authors: Nuez-Martínez M, Pedrosa L, Martinez-Rovira I, Yousef I, Diao D, Teixidor F, Stanzani E, Martínez-Soler F, Tortosa A, Sierra À, Gonzalez JJ, Viñas C
- Issue date: 2021 Sep 14
- Synchrotron- and focal plane array-based Fourier-transform infrared spectroscopy differentiates the basalis and functionalis epithelial endometrial regions and identifies putative stem cell regions of human endometrial glands.
- Authors: Theophilou G, Morais CLM, Halliwell DE, Lima KMG, Drury J, Martin-Hirsch PL, Stringfellow HF, Hapangama DK, Martin FL
- Issue date: 2018 Jul
- Fourier transform infrared (FTIR) spectromicroscopic characterization of stem-like cell populations in human esophageal normal and adenocarcinoma cell lines.
- Authors: Zhao R, Quaroni L, Casson AG
- Issue date: 2010 Jan
- Evidence for a stem-cell lineage in corneal squamous cell carcinoma using synchrotron-based Fourier-transform infrared microspectroscopy and multivariate analysis.
- Authors: Kelly JG, Nakamura T, Kinoshita S, Fullwood NJ, Martin FL
- Issue date: 2010 Dec