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dc.contributor.authorHoggard, Nigel
dc.contributor.authorHey, Yvonne
dc.contributor.authorBrintnell, Bill
dc.contributor.authorJames, Louise A
dc.contributor.authorJones, David
dc.contributor.authorMitchell, Erika L D
dc.contributor.authorWeissenbach, J
dc.contributor.authorVarley, Jennifer
dc.date.accessioned2010-12-10T12:56:12Z
dc.date.available2010-12-10T12:56:12Z
dc.date.issued1995-11-20
dc.identifier.citationIdentification and cloning in yeast artificial chromosomes of a region of elevated loss of heterozygosity on chromosome 1p31.1 in human breast cancer. 1995, 30 (2):233-43 Genomicsen
dc.identifier.issn0888-7543
dc.identifier.pmid8586422
dc.identifier.doi10.1006/geno.1995.9882
dc.identifier.urihttp://hdl.handle.net/10541/117668
dc.description.abstractWe have mapped a region of high loss of heterozygosity in breast cancer to a 2-cM interval between the loci D1S430 and D1S465 on chromosome 1p31.1. This region shows allelic imbalance in around 60% of breast tumors. As part of a strategy to clone the target gene(s) within this interval, we have generated a yeast artificial chromosome contig spanning over 7 Mb. YACs from the CEPH and Zeneca (formerly ICI) libraries have been obtained by screening with PCR-based STSs from the region for both previously identified loci and newly isolated STSs. The YACs have been assembled into a contig by a combination of approaches, including analysis of their STS content, generation of new STSs from the ends of key YACs, and long-range restriction mapping. These YAC clones provide the basis for complete characterization of the region of high loss in breast cancer and for the ultimate identification of the target gene(s).
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshBase Sequence
dc.subject.meshBreast Neoplasms
dc.subject.meshChromosome Deletion
dc.subject.meshChromosome Mapping
dc.subject.meshChromosomes, Artificial, Yeast
dc.subject.meshChromosomes, Human, Pair 1
dc.subject.meshCloning, Molecular
dc.subject.meshDNA Primers
dc.subject.meshHeterozygote
dc.subject.meshHumans
dc.subject.meshIn Situ Hybridization, Fluorescence
dc.subject.meshMolecular Sequence Data
dc.subject.meshSequence Tagged Sites
dc.titleIdentification and cloning in yeast artificial chromosomes of a region of elevated loss of heterozygosity on chromosome 1p31.1 in human breast cancer.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Manchester, United Kingdom.en
dc.identifier.journalGenomicsen
html.description.abstractWe have mapped a region of high loss of heterozygosity in breast cancer to a 2-cM interval between the loci D1S430 and D1S465 on chromosome 1p31.1. This region shows allelic imbalance in around 60% of breast tumors. As part of a strategy to clone the target gene(s) within this interval, we have generated a yeast artificial chromosome contig spanning over 7 Mb. YACs from the CEPH and Zeneca (formerly ICI) libraries have been obtained by screening with PCR-based STSs from the region for both previously identified loci and newly isolated STSs. The YACs have been assembled into a contig by a combination of approaches, including analysis of their STS content, generation of new STSs from the ends of key YACs, and long-range restriction mapping. These YAC clones provide the basis for complete characterization of the region of high loss in breast cancer and for the ultimate identification of the target gene(s).


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