Identification and cloning in yeast artificial chromosomes of a region of elevated loss of heterozygosity on chromosome 1p31.1 in human breast cancer.
Authors
Hoggard, NigelHey, Yvonne
Brintnell, Bill
James, Louise A
Jones, David
Mitchell, Erika L D
Weissenbach, J
Varley, Jennifer
Affiliation
CRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Manchester, United Kingdom.Issue Date
1995-11-20
Metadata
Show full item recordAbstract
We have mapped a region of high loss of heterozygosity in breast cancer to a 2-cM interval between the loci D1S430 and D1S465 on chromosome 1p31.1. This region shows allelic imbalance in around 60% of breast tumors. As part of a strategy to clone the target gene(s) within this interval, we have generated a yeast artificial chromosome contig spanning over 7 Mb. YACs from the CEPH and Zeneca (formerly ICI) libraries have been obtained by screening with PCR-based STSs from the region for both previously identified loci and newly isolated STSs. The YACs have been assembled into a contig by a combination of approaches, including analysis of their STS content, generation of new STSs from the ends of key YACs, and long-range restriction mapping. These YAC clones provide the basis for complete characterization of the region of high loss in breast cancer and for the ultimate identification of the target gene(s).Citation
Identification and cloning in yeast artificial chromosomes of a region of elevated loss of heterozygosity on chromosome 1p31.1 in human breast cancer. 1995, 30 (2):233-43 GenomicsJournal
GenomicsDOI
10.1006/geno.1995.9882PubMed ID
8586422Type
ArticleLanguage
enISSN
0888-7543ae974a485f413a2113503eed53cd6c53
10.1006/geno.1995.9882