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dc.contributor.authorYamazaki, K
dc.contributor.authorEyden, Brian P
dc.date.accessioned2010-12-10T14:41:01Z
dc.date.available2010-12-10T14:41:01Z
dc.date.issued1996-07
dc.identifier.citationUltrastructural and immunohistochemical studies of stromal cells in lamina propria of human fallopian tube ampullar mucosa: the recognition of 'CD34 positive reticular network' and its putative function for immune surveillance. 1996, 28 (3):325-37 J Submicrosc Cytol Patholen
dc.identifier.issn1122-9497
dc.identifier.pmid8765577
dc.identifier.urihttp://hdl.handle.net/10541/117660
dc.description.abstractThis paper aims at clarifying the cellular differentiation at the ultrastructural and immunohistochemical levels in normal stromal cells of the fallopian tube ampullar mucosa in order to arrive at a functional interpretation of these cells. Techniques used were histology, histochemistry, transmission and scanning electron microscopy, as well as light and ultrastructural immunohistochemistry for the CD34 antigen. Three types of stromal mesenchymal cell were identified. The most frequent cell-type had a fibroblastic shape and lacked the lamina and myofilaments typical of smooth-muscle. It was, however, positive for alpha-smooth-muscle actin (alpha-SMA). It was negative for desmin (D), but positive for the CD34 antigen. This cell was therefore rather undifferentiated ultrastructurally but had a partial smooth-muscle immunophenotype: it was designated as an ultrastructurally undifferentiated smooth-muscle cell (U-SM cell). A second category consisted of overt smooth-muscle cells (SM cells): they were rich in myofilaments, had a lamina and were D(+) and alpha-SMA(+). The third category resembled SM cells but were D(-): these were designated D(-) SM cells. U-SM cells, SM cells and D(-)SM cells accounted for 83%, 13% and 4% of the total stromal cell population respectively. U-SM cells had lipid-rich residual bodies, solitary cilia, simple intercellular and cell-to-matrix junction, and they were frequently adherent to mononuclear cells. This phenotype was present irrespective of the varied clinical picture of the patients from whom samples were obtained. The observations suggest that the stroma of the fallopian tube ampullar mucosa consists of a reticulum composed largely of CD34(+) U-SM cells in combination with mononuclear cells. One of the main roles suggested for this CD34 positive reticular network is immune surveillance.
dc.language.isoenen
dc.subject.meshAcid Phosphatase
dc.subject.meshActins
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshAntigens, CD34
dc.subject.meshAntigens, CD45
dc.subject.meshBiological Markers
dc.subject.meshCathepsin D
dc.subject.meshCell Adhesion
dc.subject.meshCell Differentiation
dc.subject.meshChild, Preschool
dc.subject.meshCollagen
dc.subject.meshConnective Tissue
dc.subject.meshDesmin
dc.subject.meshFallopian Tubes
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshImmunologic Surveillance
dc.subject.meshInfant, Newborn
dc.subject.meshMicroscopy, Electron, Scanning
dc.subject.meshMicroscopy, Immunoelectron
dc.subject.meshMiddle Aged
dc.subject.meshPhenotype
dc.subject.meshvon Willebrand Factor
dc.titleUltrastructural and immunohistochemical studies of stromal cells in lamina propria of human fallopian tube ampullar mucosa: the recognition of 'CD34 positive reticular network' and its putative function for immune surveillance.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology, School of Medicine Keio University, Tokyo, Japan.en
dc.identifier.journalJournal of Submicroscopic Cytology and Pathologyen
html.description.abstractThis paper aims at clarifying the cellular differentiation at the ultrastructural and immunohistochemical levels in normal stromal cells of the fallopian tube ampullar mucosa in order to arrive at a functional interpretation of these cells. Techniques used were histology, histochemistry, transmission and scanning electron microscopy, as well as light and ultrastructural immunohistochemistry for the CD34 antigen. Three types of stromal mesenchymal cell were identified. The most frequent cell-type had a fibroblastic shape and lacked the lamina and myofilaments typical of smooth-muscle. It was, however, positive for alpha-smooth-muscle actin (alpha-SMA). It was negative for desmin (D), but positive for the CD34 antigen. This cell was therefore rather undifferentiated ultrastructurally but had a partial smooth-muscle immunophenotype: it was designated as an ultrastructurally undifferentiated smooth-muscle cell (U-SM cell). A second category consisted of overt smooth-muscle cells (SM cells): they were rich in myofilaments, had a lamina and were D(+) and alpha-SMA(+). The third category resembled SM cells but were D(-): these were designated D(-) SM cells. U-SM cells, SM cells and D(-)SM cells accounted for 83%, 13% and 4% of the total stromal cell population respectively. U-SM cells had lipid-rich residual bodies, solitary cilia, simple intercellular and cell-to-matrix junction, and they were frequently adherent to mononuclear cells. This phenotype was present irrespective of the varied clinical picture of the patients from whom samples were obtained. The observations suggest that the stroma of the fallopian tube ampullar mucosa consists of a reticulum composed largely of CD34(+) U-SM cells in combination with mononuclear cells. One of the main roles suggested for this CD34 positive reticular network is immune surveillance.


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