Ultrastructural and immunohistochemical studies of stromal cells in lamina propria of human fallopian tube ampullar mucosa: the recognition of 'CD34 positive reticular network' and its putative function for immune surveillance.
AffiliationDepartment of Pathology, School of Medicine Keio University, Tokyo, Japan.
MetadataShow full item record
AbstractThis paper aims at clarifying the cellular differentiation at the ultrastructural and immunohistochemical levels in normal stromal cells of the fallopian tube ampullar mucosa in order to arrive at a functional interpretation of these cells. Techniques used were histology, histochemistry, transmission and scanning electron microscopy, as well as light and ultrastructural immunohistochemistry for the CD34 antigen. Three types of stromal mesenchymal cell were identified. The most frequent cell-type had a fibroblastic shape and lacked the lamina and myofilaments typical of smooth-muscle. It was, however, positive for alpha-smooth-muscle actin (alpha-SMA). It was negative for desmin (D), but positive for the CD34 antigen. This cell was therefore rather undifferentiated ultrastructurally but had a partial smooth-muscle immunophenotype: it was designated as an ultrastructurally undifferentiated smooth-muscle cell (U-SM cell). A second category consisted of overt smooth-muscle cells (SM cells): they were rich in myofilaments, had a lamina and were D(+) and alpha-SMA(+). The third category resembled SM cells but were D(-): these were designated D(-) SM cells. U-SM cells, SM cells and D(-)SM cells accounted for 83%, 13% and 4% of the total stromal cell population respectively. U-SM cells had lipid-rich residual bodies, solitary cilia, simple intercellular and cell-to-matrix junction, and they were frequently adherent to mononuclear cells. This phenotype was present irrespective of the varied clinical picture of the patients from whom samples were obtained. The observations suggest that the stroma of the fallopian tube ampullar mucosa consists of a reticulum composed largely of CD34(+) U-SM cells in combination with mononuclear cells. One of the main roles suggested for this CD34 positive reticular network is immune surveillance.
CitationUltrastructural and immunohistochemical studies of stromal cells in lamina propria of human fallopian tube ampullar mucosa: the recognition of 'CD34 positive reticular network' and its putative function for immune surveillance. 1996, 28 (3):325-37 J Submicrosc Cytol Pathol
JournalJournal of Submicroscopic Cytology and Pathology
- Ultrastructural and immunohistochemical studies of intralobular fibroblasts in human submandibular gland: the recognition of a 'CD34 positive reticular network' connected by gap junctions.
- Authors: Yamazaki K, Eyden BP
- Issue date: 1996 Oct
- Expression of CD34, alpha-smooth muscle actin, and transforming growth factor beta1 in squamous intraepithelial lesions and squamous cell carcinoma of the larynx and hypopharynx.
- Authors: Kojc N, Zidar N, Vodopivec B, Gale N
- Issue date: 2005 Jan
- Immunostaining of vascular, perivascular cells and stromal components in human placental villi.
- Authors: Kacemi A, Vervelle C, Uzan S, Challier JC
- Issue date: 1999 Feb
- Comparative study of CD34, alpha-SMA and h-caldesmon expression in the stroma of gynaecomastia and male breast carcinoma.
- Authors: Kalekou H, Kostopoulos I, Milias S, Papadimitriou CS
- Issue date: 2005 Jul
- Undifferentiated (embryonal) sarcoma of the liver in middle-aged adults: smooth muscle differentiation determined by immunohistochemistry and electron microscopy.
- Authors: Nishio J, Iwasaki H, Sakashita N, Haraoka S, Isayama T, Naito M, Miyayama H, Yamashita Y, Kikuchi M
- Issue date: 2003 Mar