Recruitment of cells in the small intestine into rapid cell cycle by small doses of external gamma or internal beta-radiation.
AffiliationPaterson Laboratories, CHristie Hospital and Holt Radium Institute, Manchester M20 9BX
MetadataShow full item record
AbstractEpithelial cell recruitment was examined in mouse ileum after external gamma-irradiation (50 cGy) or internal beta-irradiation (0.148 MBq/g of [3H]thymidine), using the per cent-labelled-mitoses method and by analysing the distribution of mitotic cells in the crypts. In the presumptive stem cell zone at the lower cell positions of the crypt, the slowly cycling cells decreased their cell cycle 6 or 12 hours after a dose of 50 cGy. In the higher cell positions, a slight shortening of the cell cycle was also observed. After administration of a high dose of [3H]thymidine, dormant (G0) cells also entered the cell cycle in the lower cell positions. The results suggest that stem cells in the crypt may react to irradiation in two ways: first, by shortening the cell cycle in cycling cells; secondly, by an entry into the cell cycle by other dormant cells. There was destruction of some cycling stem cells before any recruitment. The data support the idea that the stem cell population in the crypt is heterogeneous.
CitationRecruitment of cells in the small intestine into rapid cell cycle by small doses of external gamma or internal beta-radiation. 1985, 48 (3):361-9 Int J Radiat Biol Relat Stud Phys Chem Med
JournalInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
- The recruitability and cell-cycle state of intestinal stem cells.
- Authors: Potten CS, Chadwick C, Ijiri K, Tsubouchi S, Hanson WR
- Issue date: 1984 Mar
- The re-establishment of hypersensitive cells in the crypts of irradiated mouse intestine.
- Authors: Ijiri K, Potten CS
- Issue date: 1984 Nov
- Influence of irradiation or thymidine (TdR) on the pattern of 3H-TdR incorporation at each cell position in the crypts of the small intestine of the mouse.
- Authors: Chwalinski S, Potten CS
- Issue date: 1987 Feb
- The relationship between ionizing radiation-induced apoptosis and stem cells in the small and large intestine.
- Authors: Potten CS, Grant HK
- Issue date: 1998 Oct
- Apoptosis in small intestinal epithelial from p53-null mice: evidence for a delayed, p53-independent G2/M-associated cell death after gamma-irradiation.
- Authors: Merritt AJ, Allen TD, Potten CS, Hickman JA
- Issue date: 1997 Jun 12