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    SCE induction and harlequin staining in mycoplasma-contaminated Chinese hamster cells.

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    Authors
    White, Gavin R M
    Ockey, Charles H
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester, M20 9BX, Great Britain
    Issue Date
    1985
    
    Metadata
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    Abstract
    Chinese hamster V79 and CHO cells infected with Mycoplasma hyorhinis show elevated sister-chromatid exchange (SCE) levels but normal cell proliferation and levels of chromosomal aberrations when compared with uninfected cells. Harlequin staining patterns differ from those seen with uninfected cells at similar levels of bromodeoxyuridine (BrdUrd), indicating that BrdUrd is rapidly depleted from the medium by the mycoplasmal uridine phosphorylase and therefore becomes unavailable over the two cell cycles necessary for harlequin staining. Continuous treatment with the antibiotic minocycline restores the SCE level and harlequin staining to that seen in uncontaminated cells. The results suggest that mycoplasma infection should be suspected if harlequin staining patterns indicate a sudden decrease in incorporation of BrdUrd in cells grown in normal levels of BrdUrd.
    Citation
    SCE induction and harlequin staining in mycoplasma-contaminated Chinese hamster cells. 1985, 93 (2):165-8 Chromosoma
    Journal
    Chromosoma
    URI
    http://hdl.handle.net/10541/117350
    DOI
    10.1007/BF00293164
    PubMed ID
    2417788
    Type
    Article
    Language
    en
    ISSN
    0009-5915
    ae974a485f413a2113503eed53cd6c53
    10.1007/BF00293164
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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