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dc.contributor.authorJack, Elspeth M
dc.contributor.authorHarrison, Christine J
dc.contributor.authorAllen, Terence D
dc.contributor.authorHarris, R
dc.date.accessioned2010-12-07T17:23:37Z
dc.date.available2010-12-07T17:23:37Z
dc.date.issued1985
dc.identifier.citationThe structural basis for C-banding. A scanning electron microscopy study. 1985, 91 (5):363-8 Chromosomaen
dc.identifier.issn0009-5915
dc.identifier.pmid3996103
dc.identifier.doi10.1007/BF00291009
dc.identifier.urihttp://hdl.handle.net/10541/117339
dc.description.abstractThe same C-banded human polymorphic chromosomes were observed in the light microscope (LM) and then in the scanning electron microscope (SEM) to investigate the structural changes produced by the C-banding technique. C-banded regions, which stained positively in LM, were highly condensed with tightly packed chromatin fibres, resembling non-banded chromosomes. In striking contrast, adjacent non-C-banded regions were represented by loosely arranged fibres, resembling G-banded chromosomes. The significance of these observations in relation to current theories on the effects of C-banding on chromosome structure is discussed.
dc.language.isoenen
dc.subject.meshAzure Stains
dc.subject.meshCentromere
dc.subject.meshChromosome Banding
dc.subject.meshChromosomes
dc.subject.meshChromosomes, Human, 1-3
dc.subject.meshChromosomes, Human, 16-18
dc.subject.meshChromosomes, Human, 6-12 and X
dc.subject.meshHeterochromatin
dc.subject.meshHot Temperature
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMicroscopy, Electron, Scanning
dc.subject.meshPolymorphism, Genetic
dc.subject.meshY Chromosome
dc.titleThe structural basis for C-banding. A scanning electron microscopy study.en
dc.typeArticleen
dc.contributor.departmentDepartment of Ultrastructure and Department of Cell Biology, Paterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester M20 9BX, UKen
dc.identifier.journalChromosomaen
html.description.abstractThe same C-banded human polymorphic chromosomes were observed in the light microscope (LM) and then in the scanning electron microscope (SEM) to investigate the structural changes produced by the C-banding technique. C-banded regions, which stained positively in LM, were highly condensed with tightly packed chromatin fibres, resembling non-banded chromosomes. In striking contrast, adjacent non-C-banded regions were represented by loosely arranged fibres, resembling G-banded chromosomes. The significance of these observations in relation to current theories on the effects of C-banding on chromosome structure is discussed.


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