The structural basis for C-banding. A scanning electron microscopy study.
AffiliationDepartment of Ultrastructure and Department of Cell Biology, Paterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester M20 9BX, UK
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AbstractThe same C-banded human polymorphic chromosomes were observed in the light microscope (LM) and then in the scanning electron microscope (SEM) to investigate the structural changes produced by the C-banding technique. C-banded regions, which stained positively in LM, were highly condensed with tightly packed chromatin fibres, resembling non-banded chromosomes. In striking contrast, adjacent non-C-banded regions were represented by loosely arranged fibres, resembling G-banded chromosomes. The significance of these observations in relation to current theories on the effects of C-banding on chromosome structure is discussed.
CitationThe structural basis for C-banding. A scanning electron microscopy study. 1985, 91 (5):363-8 Chromosoma