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dc.contributor.authorRoberts, Ruth A
dc.contributor.authorGallagher, John T
dc.contributor.authorSpooncer, Elaine
dc.contributor.authorAllen, Terence D
dc.contributor.authorBloomfield, F
dc.contributor.authorDexter, T Michael
dc.date.accessioned2010-12-03T16:40:11Z
dc.date.available2010-12-03T16:40:11Z
dc.date.issued1988-03-24
dc.identifier.citationHeparan sulphate bound growth factors: a mechanism for stromal cell mediated haemopoiesis. 1988, 332 (6162):376-8 Natureen
dc.identifier.issn0028-0836
dc.identifier.pmid2965305
dc.identifier.doi10.1038/332376a0
dc.identifier.urihttp://hdl.handle.net/10541/117156
dc.description.abstractThe proliferation and development of haemopoietic stem cells takes place in close association with marrow stromal cells. This intimate cell contact presumably enables the stem cells and their progeny to respond to stimuli present on the stromal cell surface. While the nature of these stimuli has not been determined, it is likely that growth factors play some role. Recently, it was demonstrated that the natural and the recombinant haemopoietic growth factor, granulocyte/macrophage colony stimulating factor (GM-CSF), could be adsorbed out of solution by an extract of human marrow stromal extracellular matrix (ECM) with retention of biological activity. However, the precise ECM molecules involved were not identified. Here, we clearly demonstrate that the major sulphated glycosaminoglycan of mouse marrow stroma, heparan sulphate, possesses the ability to adsorb both GM-CSF and the multilineage haemopoietic growth factor, Interleukin 3 (IL-3). Furthermore, these growth factors, once bound, can be presented in the biologically active form to haemopoietic cells.
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAdsorption
dc.subject.meshAnimals
dc.subject.meshBone Marrow Cells
dc.subject.meshCells, Cultured
dc.subject.meshColony-Stimulating Factors
dc.subject.meshExtracellular Matrix
dc.subject.meshGlycosaminoglycans
dc.subject.meshHematopoiesis
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHeparitin Sulfate
dc.subject.meshInterleukin-3
dc.subject.meshMice
dc.subject.meshProtein Binding
dc.titleHeparan sulphate bound growth factors: a mechanism for stromal cell mediated haemopoiesis.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Haematology, Christie Hospital and Holt Radium Institute, Withington, Manchester, UK.en
dc.identifier.journalNatureen
html.description.abstractThe proliferation and development of haemopoietic stem cells takes place in close association with marrow stromal cells. This intimate cell contact presumably enables the stem cells and their progeny to respond to stimuli present on the stromal cell surface. While the nature of these stimuli has not been determined, it is likely that growth factors play some role. Recently, it was demonstrated that the natural and the recombinant haemopoietic growth factor, granulocyte/macrophage colony stimulating factor (GM-CSF), could be adsorbed out of solution by an extract of human marrow stromal extracellular matrix (ECM) with retention of biological activity. However, the precise ECM molecules involved were not identified. Here, we clearly demonstrate that the major sulphated glycosaminoglycan of mouse marrow stroma, heparan sulphate, possesses the ability to adsorb both GM-CSF and the multilineage haemopoietic growth factor, Interleukin 3 (IL-3). Furthermore, these growth factors, once bound, can be presented in the biologically active form to haemopoietic cells.


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