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dc.contributor.authorSimmons, P J
dc.contributor.authorLord, Brian I
dc.date.accessioned2010-12-03T12:07:44Z
dc.date.available2010-12-03T12:07:44Z
dc.date.issued1985-10
dc.identifier.citationEnrichment of CFU-S proliferation inhibitor-producing cells based on their identification by the monoclonal antibody F4/80. 1985, 78:117-31 J Cell Scien
dc.identifier.issn0021-9533
dc.identifier.pmid4093468
dc.identifier.urihttp://hdl.handle.net/10541/117091
dc.description.abstractCells capable of synthesizing a factor that can specifically inhibit the proliferation of haemopoietic spleen colony-forming units, CFU-S, carry many of the characteristics of macrophages. The monoclonal antibody, F4/80, which is specific for murine macrophages, has therefore been used to isolate macrophages from bone marrow. These macrophages were then assayed for their ability to produce the inhibitor. Low-density bone marrow cells were first separated by a density-cut procedure and then labelled with F4/80. Fluorescence-activated cell sorting was then used to select F4/80 positive and negative cell fractions. It was found that the F4/80 positive fraction contains inhibitor cells, enriched by at least 200-fold compared to unfractionated marrow. Subsequent culture of this fraction over a period of several weeks produced a further 15- to 20-fold increase in inhibitor-producing capacity. The cultured cells producing inhibitor were virtually 100% F4/80 positive, phagocytic and exhibited histochemical properties characteristic of macrophages. The macrophage-like character of the producer cells was thus confirmed and the removal of the majority of unwanted cells means that many of the impurities in the normal crude extracts have been excluded.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals
dc.subject.meshAntibodies, Monoclonal
dc.subject.meshBone Marrow Cells
dc.subject.meshCell Division
dc.subject.meshCell Separation
dc.subject.meshCells, Cultured
dc.subject.meshFlow Cytometry
dc.subject.meshGrowth Inhibitors
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshMacrophages
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshSpleen
dc.titleEnrichment of CFU-S proliferation inhibitor-producing cells based on their identification by the monoclonal antibody F4/80.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester, M20 9BX, UK.en
dc.identifier.journalJournal of Cell Scienceen
html.description.abstractCells capable of synthesizing a factor that can specifically inhibit the proliferation of haemopoietic spleen colony-forming units, CFU-S, carry many of the characteristics of macrophages. The monoclonal antibody, F4/80, which is specific for murine macrophages, has therefore been used to isolate macrophages from bone marrow. These macrophages were then assayed for their ability to produce the inhibitor. Low-density bone marrow cells were first separated by a density-cut procedure and then labelled with F4/80. Fluorescence-activated cell sorting was then used to select F4/80 positive and negative cell fractions. It was found that the F4/80 positive fraction contains inhibitor cells, enriched by at least 200-fold compared to unfractionated marrow. Subsequent culture of this fraction over a period of several weeks produced a further 15- to 20-fold increase in inhibitor-producing capacity. The cultured cells producing inhibitor were virtually 100% F4/80 positive, phagocytic and exhibited histochemical properties characteristic of macrophages. The macrophage-like character of the producer cells was thus confirmed and the removal of the majority of unwanted cells means that many of the impurities in the normal crude extracts have been excluded.


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