Show simple item record

dc.contributor.authorMackett, Mike
dc.contributor.authorArrand, John R
dc.date.accessioned2010-12-03T11:31:55Z
dc.date.available2010-12-03T11:31:55Z
dc.date.issued1985-12-01
dc.identifier.citationRecombinant vaccinia virus induces neutralising antibodies in rabbits against Epstein-Barr virus membrane antigen gp340. 1985, 4 (12):3229-34 EMBO Jen
dc.identifier.issn0261-4189
dc.identifier.pmid3004944
dc.identifier.urihttp://hdl.handle.net/10541/117083
dc.description.abstractThe Epstein-Barr virus membrane antigen gene gp340 was isolated, inserted into several strains of vaccinia virus and expressed under the control of a vaccinia virus promoter. The EBV-derived protein which was produced by the recombinant vaccinia viruses was heavily glycosylated, readily labelled with threonine, could be detected at the surface of infected cells and had a mol. wt. of approximately 340 kd, all of which are properties of the authentic gp340. Polyclonal rabbit antisera against gp340 and an EBV-neutralising anti-gp340 monoclonal antibody both recognised cells infected with the recombinant vaccinia viruses. Moreover, rabbits vaccinated with one of the recombinants produced antibodies that recognised EBV-containing lymphoblastoid cells and neutralised EBV.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAntibodies, Viral
dc.subject.meshAntibody Formation
dc.subject.meshDNA Restriction Enzymes
dc.subject.meshFluorescent Antibody Technique
dc.subject.meshGenes
dc.subject.meshGenes, Viral
dc.subject.meshGenetic Vectors
dc.subject.meshHerpesvirus 4, Human
dc.subject.meshPlasmids
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshRabbits
dc.subject.meshThymidine Kinase
dc.subject.meshVaccinia virus
dc.subject.meshViral Envelope Proteins
dc.titleRecombinant vaccinia virus induces neutralising antibodies in rabbits against Epstein-Barr virus membrane antigen gp340.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Biology, Paterson Laboratories, Christie Hopital and Holt Radium Institute, Manchester M20 9BX, UK.en
dc.identifier.journalThe EMBO Journalen
html.description.abstractThe Epstein-Barr virus membrane antigen gene gp340 was isolated, inserted into several strains of vaccinia virus and expressed under the control of a vaccinia virus promoter. The EBV-derived protein which was produced by the recombinant vaccinia viruses was heavily glycosylated, readily labelled with threonine, could be detected at the surface of infected cells and had a mol. wt. of approximately 340 kd, all of which are properties of the authentic gp340. Polyclonal rabbit antisera against gp340 and an EBV-neutralising anti-gp340 monoclonal antibody both recognised cells infected with the recombinant vaccinia viruses. Moreover, rabbits vaccinated with one of the recombinants produced antibodies that recognised EBV-containing lymphoblastoid cells and neutralised EBV.


This item appears in the following Collection(s)

Show simple item record