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dc.contributor.authorFransson, L A
dc.contributor.authorHampson, Ian N
dc.contributor.authorKumar, Shant
dc.contributor.authorGallagher, John T
dc.date.accessioned2010-12-02T11:16:11Z
dc.date.available2010-12-02T11:16:11Z
dc.date.issued1985
dc.identifier.citationChemical heterogeneity of heparan sulfate from a human neuroblastoma cell line. 1985, 39 (4):305-13 Acta Chem Scand, B, Org Chem Biochemen
dc.identifier.issn0302-4369
dc.identifier.pmid3159173
dc.identifier.urihttp://hdl.handle.net/10541/116914
dc.description.abstractThe chemical heterogeneity of radiolabelled neuroblastoma heparan sulfate has been studied by ion exchange chromatography and by affinity chromatography on heparan sulfate-agarose. Although the entire population of chains shows considerable homogeneity in charge density, the deaminative cleavage products ranged in size from disaccharides to eicosasaccharides. Under appropriate conditions neuroblastoma heparan sulfate could be separated into two pools of low or high affinity for lung heparan sulfate-agarose. Analyses of periodate oxidation-alkaline elimination indicated that the high affinity chains contained larger proportions of heparin-like segments, i.e. iduronate-rich and N-sulfated ones.
dc.language.isoenen
dc.subject.meshCell Line
dc.subject.meshChromatography, Affinity
dc.subject.meshChromatography, Ion Exchange
dc.subject.meshGlycosaminoglycans
dc.subject.meshHeparitin Sulfate
dc.subject.meshHumans
dc.subject.meshNeuroblastoma
dc.titleChemical heterogeneity of heparan sulfate from a human neuroblastoma cell line.en
dc.typeArticleen
dc.identifier.journalActa Chemica Scandinavica. Series B: Organic Chemistry and Biochemistryen
html.description.abstractThe chemical heterogeneity of radiolabelled neuroblastoma heparan sulfate has been studied by ion exchange chromatography and by affinity chromatography on heparan sulfate-agarose. Although the entire population of chains shows considerable homogeneity in charge density, the deaminative cleavage products ranged in size from disaccharides to eicosasaccharides. Under appropriate conditions neuroblastoma heparan sulfate could be separated into two pools of low or high affinity for lung heparan sulfate-agarose. Analyses of periodate oxidation-alkaline elimination indicated that the high affinity chains contained larger proportions of heparin-like segments, i.e. iduronate-rich and N-sulfated ones.


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