Chemical heterogeneity of heparan sulfate from a human neuroblastoma cell line.
Abstract
The chemical heterogeneity of radiolabelled neuroblastoma heparan sulfate has been studied by ion exchange chromatography and by affinity chromatography on heparan sulfate-agarose. Although the entire population of chains shows considerable homogeneity in charge density, the deaminative cleavage products ranged in size from disaccharides to eicosasaccharides. Under appropriate conditions neuroblastoma heparan sulfate could be separated into two pools of low or high affinity for lung heparan sulfate-agarose. Analyses of periodate oxidation-alkaline elimination indicated that the high affinity chains contained larger proportions of heparin-like segments, i.e. iduronate-rich and N-sulfated ones.Citation
Chemical heterogeneity of heparan sulfate from a human neuroblastoma cell line. 1985, 39 (4):305-13 Acta Chem Scand, B, Org Chem BiochemJournal
Acta Chemica Scandinavica. Series B: Organic Chemistry and BiochemistryPubMed ID
3159173Type
ArticleLanguage
enISSN
0302-4369Related articles
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