Development of spleen CFU-S colonies from day 8 to day 11: relationship to self-renewal capacity.
MetadataShow full item record
AbstractTo investigate the persistence of spleen colonies from day 8 to day 11 of their development, we injected low numbers of marrow cells in order to obtain single colonies on the spleens of irradiated mice. Colonies were isolated on either half of the spleen on the eighth day. The position of day-11 colonies, determined relative to the ligature, indicated where novel colonies appear between those times. The results showed no evidence of the persistence of colonies from day 8 to day 11. The self-reproduction capacity of CFU-S that survive various cytotoxic drugs depends on the specific subpopulations that are affected by the drug. Using cyclophosphamide, busulphan, or BCNU, the self-renewal capacity of surviving CFU-S was manipulated. The results show that after cytotoxic treatments, a high day-11-day-8 ratio is not necessarily a reflection of a high self-renewal capacity of the CFU-S population that forms the day-11 colonies.
CitationDevelopment of spleen CFU-S colonies from day 8 to day 11: relationship to self-renewal capacity. 1986, 14 (8):710-3 Exp Hematol
- Heterogeneity within the spleen colony-forming cell population in rat bone marrow.
- Authors: Martens AC, van Bekkum DW, Hagenbeek A
- Issue date: 1986 Sep
- On the late seeding of CFU-S to the spleen: 8- vs 12-day CFU-S.
- Authors: Lord BI, Molineux G, Schofield R, Humphreys ER, Stones VA
- Issue date: 1989 Aug
- Isolation of hemopoietic stem cell subsets from murine bone marrow: I. Radioprotective ability of purified cell suspensions differing in the proportion of day-7 and day-12 CFU-S.
- Authors: Ploemacher RE, Brons NH
- Issue date: 1988 Jan
- Kinetics of early and late spleen colony development.
- Authors: Wolf NS, Priestley GV
- Issue date: 1986 Aug
- Separation and functional analysis of bone marrow cells separated by rhodamine-123 fluorescence.
- Authors: Mulder AH, Visser JW
- Issue date: 1987 Jan