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dc.contributor.authorHendry, Jolyon H
dc.contributor.authorSchofield, Raymond
dc.contributor.authorBwire, N
dc.date.accessioned2010-11-30T18:25:24Z
dc.date.available2010-11-30T18:25:24Z
dc.date.issued1986-03
dc.identifier.citationRadiosensitivity of murine hemopoietic colony-forming units assayed in situ in the rib and in other marrow sites. 1986, 105 (3):370-8 Radiat Resen
dc.identifier.issn0033-7587
dc.identifier.pmid3961099
dc.identifier.urihttp://hdl.handle.net/10541/116795
dc.description.abstractThe radiosensitivity of murine hemopoietic colony-forming cells, which produce colonies in situ and which were counted at Day 8 after irradiation in sections of the femur, humerus, sternum, and spleen, is characterized by a D0 value of 91 +/- 9 cGy. The radiosensitivity of such cells in the rib was assessed using a new technique measuring regeneration or ablation of marrow in transverse sections of ribs observed at Day 8 after irradiation. The mean D0 value over a range determined using several different criteria was 108 cGy. These results provide evidence for the common assumptions that radiosensitivity measured using conventional transplantation assays reflects radiosensitivity in situ, and that the radiosensitivity of stem cells in different medullary marrow sites is similar. The techniques could be used with other species where assays for stem cells are not available.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals
dc.subject.meshCesium Radioisotopes
dc.subject.meshColony-Forming Units Assay
dc.subject.meshDose-Response Relationship, Radiation
dc.subject.meshFemale
dc.subject.meshFemur
dc.subject.meshGamma Rays
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHumerus
dc.subject.meshLumbar Vertebrae
dc.subject.meshMice
dc.subject.meshRadiation Tolerance
dc.subject.meshRibs
dc.subject.meshSpleen
dc.subject.meshSternum
dc.titleRadiosensitivity of murine hemopoietic colony-forming units assayed in situ in the rib and in other marrow sites.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, UK.en
dc.identifier.journalRadiation Researchen
html.description.abstractThe radiosensitivity of murine hemopoietic colony-forming cells, which produce colonies in situ and which were counted at Day 8 after irradiation in sections of the femur, humerus, sternum, and spleen, is characterized by a D0 value of 91 +/- 9 cGy. The radiosensitivity of such cells in the rib was assessed using a new technique measuring regeneration or ablation of marrow in transverse sections of ribs observed at Day 8 after irradiation. The mean D0 value over a range determined using several different criteria was 108 cGy. These results provide evidence for the common assumptions that radiosensitivity measured using conventional transplantation assays reflects radiosensitivity in situ, and that the radiosensitivity of stem cells in different medullary marrow sites is similar. The techniques could be used with other species where assays for stem cells are not available.


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