Studies of the enhancement of natural cytotoxicity by the streptococcal immunopotentiator OK432.
AffiliationDepartment of Immunology, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, UK.
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AbstractThe immunopotentiator OK432, a product of Streptococcus pyogenes A3, enhanced the natural killer (NK) activity of unseparated human peripheral blood mononuclear cells (PBM) and separated, nonadherent lymphocytes (PBL) containing less than 0.5% contamination with monocytes. Following treatment with OK432, both unseparated and non-adherent cell preparations produced interferon (IFN) alpha and gamma and low levels of interleukin-2 (IL-2). However, in the presence of neutralising amounts of anti-IFN alpha and gamma antisera, the NK enhancing effect of OK432 was not fully inhibited. Filtered supernatants derived from OK432-treated PBM or PBL also enhanced the NK activity of fresh PBM. In this instance, in most experiments, the NK enhancing effect of supernatants (from non-adherent cells) was fully inhibited by anti-IFN antisera. However, in some experiments, enhancement of NK activity by anti-IFN antisera-treated supernatants was still found. It is suggested that the presence of IL-2 or, possibly, other as yet uncharacterized factors secreted following OK432 treatment, account for this residual enhancement of NK activity.
CitationStudies of the enhancement of natural cytotoxicity by the streptococcal immunopotentiator OK432. 1986, 8 (1):83-92 Int J Immunopharmacol
JournalInternational Journal of Immunopharmacology