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dc.contributor.authorLord, Brian I
dc.date.accessioned2010-11-30T18:18:58Z
dc.date.available2010-11-30T18:18:58Z
dc.date.issued1986-05
dc.identifier.citationThe sensitivity of G0-state haemopoietic spleen colony-forming cells to a stimulus for proliferation. 1986, 19 (3):305-10 Cell Tissue Kineten
dc.identifier.issn0008-8730
dc.identifier.pmid3719662
dc.identifier.doi10.1111/j.1365-2184.1986.tb00682.x
dc.identifier.urihttp://hdl.handle.net/10541/116792
dc.description.abstractHaemopoietic spleen colony-forming units (CFU-s) close to the axis (axial CFU-s) of the long bones have a high probability of self-renewal. They are pluripotent cells and are largely in a G0-State. By contrast, CFU-s close to the bone surface (marginal CFU-s) have a lower probability of self-renewal and are probably more mature, though still pluripotent. Most CFU-s proliferation arises in this zone. As a consequence, marginal CFU-s tend to have shorter G0 histories than do axial CFU-s. Femoral marrow was, therefore, divided into axial and marginal populations and the sensitivity of the CFU-s to an endogenous CFU-s-specific proliferation-stimulating factor was assessed and compared by the tritiated thymidine suicide technique. It was found that axial CFU-s are considerably more resistant to stimulation than are marginal CFU-s in that larger doses for longer periods of exposure are required to increase the proliferative activity of the cells. This behaviour is consistent with the suggestion that cells with a low division probability exist in deeper levels of the quiescent G0-state. Although this hypothesis was developed from the behaviour of cells maintained in culture under sub-optimal physiological conditions, this phenomenon appears, in vivo, to be a characteristic of the stem cell population of haemopoietic tissue; their high resistance to stimulation maintaining the axial CFU-s in a quiescent state.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals
dc.subject.meshBone Marrow
dc.subject.meshBone Marrow Cells
dc.subject.meshCell Division
dc.subject.meshCell Survival
dc.subject.meshColony-Forming Units Assay
dc.subject.meshCulture Media
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshInterphase
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshSpleen
dc.subject.meshThymidine
dc.titleThe sensitivity of G0-state haemopoietic spleen colony-forming cells to a stimulus for proliferation.en
dc.typeArticleen
dc.contributor.departmentThe Paterson Laboratories, Wilmslow Road, Withington, Manchester, M20 9BX, UK.en
dc.identifier.journalCell and Tissue Kineticsen
html.description.abstractHaemopoietic spleen colony-forming units (CFU-s) close to the axis (axial CFU-s) of the long bones have a high probability of self-renewal. They are pluripotent cells and are largely in a G0-State. By contrast, CFU-s close to the bone surface (marginal CFU-s) have a lower probability of self-renewal and are probably more mature, though still pluripotent. Most CFU-s proliferation arises in this zone. As a consequence, marginal CFU-s tend to have shorter G0 histories than do axial CFU-s. Femoral marrow was, therefore, divided into axial and marginal populations and the sensitivity of the CFU-s to an endogenous CFU-s-specific proliferation-stimulating factor was assessed and compared by the tritiated thymidine suicide technique. It was found that axial CFU-s are considerably more resistant to stimulation than are marginal CFU-s in that larger doses for longer periods of exposure are required to increase the proliferative activity of the cells. This behaviour is consistent with the suggestion that cells with a low division probability exist in deeper levels of the quiescent G0-state. Although this hypothesis was developed from the behaviour of cells maintained in culture under sub-optimal physiological conditions, this phenomenon appears, in vivo, to be a characteristic of the stem cell population of haemopoietic tissue; their high resistance to stimulation maintaining the axial CFU-s in a quiescent state.


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