The sensitivity of G0-state haemopoietic spleen colony-forming cells to a stimulus for proliferation.
Authors
Lord, Brian IAffiliation
The Paterson Laboratories, Wilmslow Road, Withington, Manchester, M20 9BX, UK.Issue Date
1986-05
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Haemopoietic spleen colony-forming units (CFU-s) close to the axis (axial CFU-s) of the long bones have a high probability of self-renewal. They are pluripotent cells and are largely in a G0-State. By contrast, CFU-s close to the bone surface (marginal CFU-s) have a lower probability of self-renewal and are probably more mature, though still pluripotent. Most CFU-s proliferation arises in this zone. As a consequence, marginal CFU-s tend to have shorter G0 histories than do axial CFU-s. Femoral marrow was, therefore, divided into axial and marginal populations and the sensitivity of the CFU-s to an endogenous CFU-s-specific proliferation-stimulating factor was assessed and compared by the tritiated thymidine suicide technique. It was found that axial CFU-s are considerably more resistant to stimulation than are marginal CFU-s in that larger doses for longer periods of exposure are required to increase the proliferative activity of the cells. This behaviour is consistent with the suggestion that cells with a low division probability exist in deeper levels of the quiescent G0-state. Although this hypothesis was developed from the behaviour of cells maintained in culture under sub-optimal physiological conditions, this phenomenon appears, in vivo, to be a characteristic of the stem cell population of haemopoietic tissue; their high resistance to stimulation maintaining the axial CFU-s in a quiescent state.Citation
The sensitivity of G0-state haemopoietic spleen colony-forming cells to a stimulus for proliferation. 1986, 19 (3):305-10 Cell Tissue KinetJournal
Cell and Tissue KineticsDOI
10.1111/j.1365-2184.1986.tb00682.xPubMed ID
3719662Type
ArticleLanguage
enISSN
0008-8730ae974a485f413a2113503eed53cd6c53
10.1111/j.1365-2184.1986.tb00682.x
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