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Evidence for the excision repair of O6-n-butyldeoxyguanosine in human cells.

dc.contributor.authorBoyle, John M
dc.contributor.authorMargison, Geoffrey P
dc.contributor.authorSaffhill, Roy
dc.date.accessioned2010-11-30T18:17:50Z
dc.date.available2010-11-30T18:17:50Z
dc.date.issued1986-12
dc.identifier.citationEvidence for the excision repair of O6-n-butyldeoxyguanosine in human cells. 1986, 7 (12):1987-90 Carcinogenesisen
dc.identifier.issn0143-3334
dc.identifier.pmid3779895
dc.identifier.doi10.1093/carcin/7.12.1987
dc.identifier.urihttp://hdl.handle.net/10541/116791
dc.description.abstractThe persistence of O6-n-butyldeoxyguanosine (O6-nBudG) in DNA, the presence of O6-alkylguanine DNA alkyltransferase (AT) activity in cell extracts, and cell survival following exposure to N-n-butyl-N-nitrosourea (BNU), have been measured in normal and xeroderma pigmentosum cell strains, both transformed and untransformed. The rates of removal of O6-nBudG did not correlate with AT activity but did correlate with the ability of strains to excise bulky DNA lesions. BNU and N-methyl-N-nitrosourea dose-response curves for cell killing suggests that both AT and excision may be involved in the repair of cytotoxic lesions.
dc.language.isoenen
dc.subject.meshAlkylation
dc.subject.meshCell Line
dc.subject.meshCell Survival
dc.subject.meshDNA
dc.subject.meshDNA Repair
dc.subject.meshDeoxyguanosine
dc.subject.meshGuanosine
dc.subject.meshHumans
dc.subject.meshMethylnitrosourea
dc.subject.meshMethyltransferases
dc.subject.meshNitrosourea Compounds
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase
dc.titleEvidence for the excision repair of O6-n-butyldeoxyguanosine in human cells.en
dc.typeArticleen
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, UK.en
dc.identifier.journalCarcinogenesisen
html.description.abstractThe persistence of O6-n-butyldeoxyguanosine (O6-nBudG) in DNA, the presence of O6-alkylguanine DNA alkyltransferase (AT) activity in cell extracts, and cell survival following exposure to N-n-butyl-N-nitrosourea (BNU), have been measured in normal and xeroderma pigmentosum cell strains, both transformed and untransformed. The rates of removal of O6-nBudG did not correlate with AT activity but did correlate with the ability of strains to excise bulky DNA lesions. BNU and N-methyl-N-nitrosourea dose-response curves for cell killing suggests that both AT and excision may be involved in the repair of cytotoxic lesions.


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