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    Identification of an Epstein-Barr virus-coded thymidine kinase.

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    Authors
    Littler, Edward
    Zeuthen, J
    McBride, A
    Trøst Sørensen, E
    Powell, K
    Walsh-Arrand, Jane E
    Arrand, John R
    Affiliation
    Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, UK.
    Issue Date
    1986-08
    
    Metadata
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    Abstract
    We have demonstrated the presence of an Epstein-Barr virus (EBV)-coded thymidine kinase (TK) by producing biochemically transformed, TK-positive mammalian cell lines using either microinjection of whole EBV virions or calcium phosphate-mediated transfection of the SalI-B restriction endonuclease fragment of EBV DNA. Analysis of these cell lines showed that: (i) EBV DNA was present in the cell lines, (ii) sequences from the SalI-B restriction endonuclease fragment of EBV were expressed, (iii) a TK activity was present and (iv) a protein with antigenic cross-reactivity with the herpes simplex virus (HSV) TK was produced. The identity of the EBV TK gene was determined by demonstrating that a recombinant plasmid, which expressed the protein product of the BXLF1 open reading frame as a fusion protein, could complement TK- strains of E. coli. A comparison of the predicted amino acid sequences of the TK proteins of EBV and HSV-1 revealed significant regions of homology.
    Citation
    Identification of an Epstein-Barr virus-coded thymidine kinase. 1986, 5 (8):1959-66 EMBO J
    Journal
    The EMBO Journal
    URI
    http://hdl.handle.net/10541/116778
    PubMed ID
    3019675
    Type
    Article
    Language
    en
    ISSN
    0261-4189
    Collections
    All Paterson Institute for Cancer Research

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