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dc.contributor.authorChen, Fu-Du
dc.contributor.authorHendry, Jolyon H
dc.date.accessioned2010-11-30T17:29:57Z
dc.date.available2010-11-30T17:29:57Z
dc.date.issued1986-04
dc.identifier.citationThe radiosensitivity of microcolony- and macrocolony-forming cells in mouse tail epidermis. 1986, 59 (700):389-95 Br J Radiolen
dc.identifier.issn0007-1285
dc.identifier.pmid3697617
dc.identifier.doi10.1259/0007-1285-59-700-389
dc.identifier.urihttp://hdl.handle.net/10541/116771
dc.description.abstractA new microcolony technique is described for measuring the survival of colony-forming cells in mouse tail epidermis. The survival curve is characterised by D0 = 2.70 +/- 0.12 Gy. The number of microcolonies per cm2 is similar to the number of macrocolonies after high doses, which shows for the first time that all microcolonies (greater than or equal to 32 cells) in epidermis develop into macrocolonies. At low doses the number of macrocolonies underestimates the number of colony-forming cells because of coalescence of microcolonies to form macrocolonies. This results in a lower apparent sensitivity of macrocolony-forming cells by a factor of about 1.5. About 3% of basal cells in tail epidermis appear to be capable of colony formation.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Count
dc.subject.meshCell Survival
dc.subject.meshDose-Response Relationship, Radiation
dc.subject.meshFemale
dc.subject.meshMice
dc.subject.meshRadiation Dosage
dc.subject.meshRadiation Tolerance
dc.subject.meshSkin
dc.subject.meshStem Cells
dc.subject.meshTail
dc.titleThe radiosensitivity of microcolony- and macrocolony-forming cells in mouse tail epidermis.en
dc.typeArticleen
dc.contributor.departmentDepartment of Radiobiology, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalThe British Journal of Radiologyen
html.description.abstractA new microcolony technique is described for measuring the survival of colony-forming cells in mouse tail epidermis. The survival curve is characterised by D0 = 2.70 +/- 0.12 Gy. The number of microcolonies per cm2 is similar to the number of macrocolonies after high doses, which shows for the first time that all microcolonies (greater than or equal to 32 cells) in epidermis develop into macrocolonies. At low doses the number of macrocolonies underestimates the number of colony-forming cells because of coalescence of microcolonies to form macrocolonies. This results in a lower apparent sensitivity of macrocolony-forming cells by a factor of about 1.5. About 3% of basal cells in tail epidermis appear to be capable of colony formation.


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